Preparation and evaluation of a bivalent peste des petits ruminants and Brucella Rev-1 vaccine for sheep

Abstract

Sheep industry is of great importance in the economy. Infectious diseases of ruminants are an economic threat and can cause massive damage globally. Peste des petits ruminants and brucellosis are two diseases that affect sheep and cause great economic losses. The live attenuated peste des petits ruminants vaccine induces strong immunity and high protection against this disease. On the other hand, the live attenuated Brucella melitensis Rev-1 vaccine represents one of the best choices for controlling Brucella diseases. In the present study, the newly prepared bivalent vaccine against peste des petits ruminants and Brucella Rev-1 from local isolates was studied in comparison with the monovalent vaccines against each disease. Enzyme-linked immunosorbent assay and seroneutralization test were used for serological evaluation of the immune response in the vaccinated sheep groups; the prepared bivalent vaccine induced higher antibody titers than the monovalent vaccines and the protective antibody titer was detected 3-4 weeks after vaccination. The bivalent vaccine can provide excellent protection to sheep against peste des petits ruminants and Brucella melitensis compared to monovalent vaccines.

Keywords

• peste des petits ruminants
• brucella
• bivalent vaccine
• neutralization test
• elisa