Inhibition of restenosis after balloon injury in rabbit vessels by integrin αvβ3-targeted 10058-F4 nanoparticles

Abstract

This study investigated whether 10058-F4, a small molecule inhibitor of the c-Myc gene, could play a corresponding inhibitory role in the vascular proliferation process. The efficacy and targeting ability of the synthesised drug-loaded nanoparticles were first measured at the cellular level, while 16 rabbit femoral arteries were then balloon damaged to establish a vascular model and treated locally with targeted αvβ3-10058-F4 nanoparticles (experimental group) and αvβ3 nanoparticles (control group), and vascular specimens were obtained after 4 weeks for analysis. The vascular specimens were found to have a vascular lumen area of 0.34 mm2 ± 0.12 mm2 in the experimental group compared to 0.79 mm2 + 0.21 mm2 in the control group (p < 0.05), and in terms of neovascular smooth muscle area, 0.22 mm2 + 0.12 mm2 in the experimental group compared to 0.64 mm2 ± 0.17 mm2 in the control group (p < 0.05), indicating a significant inhibition of vascular proliferation after drug application. The small molecule inhibitor of the c-Myc gene, 10058-F4, can play a significant inhibitory role in the process of vascular proliferation, and the locally targeted nano-delivery method allows the drug to be activated within the smooth muscle cell layer to prevent stenosis and does not retard endothelial healing.

Keywords

• Restenosis
• c-Myc inhibitor
• αvβ3 nanomedicine
• drug delivery