Terbinafine Nanohybrid: Proposing a Hydrogel Carrying Nanoparticles for Topical Release
Louise Lacalendola Tundisi,
Janaína Artem Ataide,
Jéssica Heline Lopes da Fonseca,
Luiza Aparecida Luna Silvério,
Marcelo Lancellotti,
Ana Cláudia Paiva-Santos,
Marcos Akira d’Ávila,
Daniel S. Kohane,
Priscila Gava Mazzola
Affiliations
Louise Lacalendola Tundisi
Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas 13083-871, SP, Brazil
Janaína Artem Ataide
Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas 13083-871, SP, Brazil
Jéssica Heline Lopes da Fonseca
Department of Manufacturing and Materials Engineering, School of Mechanical Engineering, University of Campinas (Unicamp), Campinas 13083-860, SP, Brazil
Luiza Aparecida Luna Silvério
Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas 13083-871, SP, Brazil
Marcelo Lancellotti
Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas 13083-871, SP, Brazil
Ana Cláudia Paiva-Santos
Department of Pharmaceutical Technology, Faculty of Pharmacy of the University of Coimbra, University of Coimbra, 3000-548 Coimbra, Portugal
Marcos Akira d’Ávila
Department of Manufacturing and Materials Engineering, School of Mechanical Engineering, University of Campinas (Unicamp), Campinas 13083-860, SP, Brazil
Daniel S. Kohane
Laboratory for Biomaterials and Drug Delivery, Department of Anaesthesiology, Division of Critical Care Medicine, Boston Children’s Hospital, Harvard Medical School, Boston, MA 02115, USA
Priscila Gava Mazzola
Faculty of Pharmaceutical Sciences, University of Campinas (Unicamp), Campinas 13083-871, SP, Brazil
A poloxamer 407 (P407)—Casein hydrogel was chosen to carry polycaprolactone nanoparticles carrying terbinafine (PCL-TBH-NP). In this study, terbinafine hydrochloride (TBH) was encapsulated into polycaprolactone (PCL) nanoparticles, which were further incorporated into a poloxamer-casein hydrogel in a different addition order to evaluate the effect of gel formation. Nanoparticles were prepared by the nanoprecipitation technique and characterized by evaluating their physicochemical characteristics and morphology. The nanoparticles had a mean diameter of 196.7 ± 0.7 nm, PDI of 0.07, negative ζ potential (−0.713 mV), high encapsulation efficiency (>98%), and did not show cytotoxic effects in primary human keratinocytes. PCL-NP modulated terbinafine was released in artificial sweat. Rheological properties were analyzed by temperature sweep tests at different addition orders of nanoparticles into hydrogel formation. The rheological behavior of nanohybrid hydrogels showed the influence of TBH-PCL nanoparticles addition in the mechanical properties of the hydrogel and a long-term release of the nanoparticles from it.
