Differences of antigenic profiles on immunoblotting of wild type measles virus and vaccine virus in Indonesia

Abstract

Background Measles virus has a negative, single strand RNA genome which codes for six important structural proteins. The genes of the wild type measles virus have many variances hence the nucleotide sequences of each wild type virus and vaccine virus are different. This differences lead to the antigenic differences between wild type and vaccine virus. Objective The purpose of this research is to investigate the differences in the antigenic profiles on immunoblotting between wild type and vaccine virus. Results The analysis results are 1) the antigen ofCAM-70 vaccine virus was less able in cross reacting with the antibodies from G2, G3, 09, CAM-70 and Schwarz; 2) The antibody aga inst CAM- 70 was only able to cross react with antigens of N protein and a few of antigens ofF proteins; 3) The wild type virus were very immunogenic, hence the antibody titers were very high; 4) The CAM-70 and MMR vaccine virus were less immunogenic, hence their antibody were very low; 5) The antibody responses that always occurred from all immunized mice serum were antibody for N and F proteins. However, the antibody against CAM-70 vaccine virus was still able to react with wild type virus (G2, G3 and 09). Conclusion All antigen-antibody reaction on immunoblotting resulted in different profiles especially between wild type virus and CAM-70 vaccine virus. Although CAM-70 vaccine virus showed clear differences compared to G2, G3 and 09 genotypes, antibodies against CAM-70 were still able to cross react with antigens from other genotypes (G2, G3 and D9).

Keywords

• measles virus
• wild type virus
• CAM-70 vaccine virus