Assessment of genetic fidelity of Fritillaria dagana (Liliaceae) regenerated plants using ISSR markers

Abstract

ISSR analysis of Fritillaria dagana, endangered ornamental geophyte from the Sayan Mountains, regenerated through direct gemmogenesis from bulb scale tissue was performed. More informative electrophoresis profile with clear and distinct bands was obtained at amplification with (CAC)3GC primer at 56 °C annealing. The genetic fidelity of F.dagana regenerants to maternal plants was confirmed.