Sedative–hypnotic effect of YZG-330 and its effect on chloride influx in mouse brain cortical cells

Abstract

This study was to examine the sedative–hypnotic effect of YZG-330 and its influence on Cl− influx in mouse cortical cells. In a sleep time-prolongation test in which mice were administered a threshold dosage of sodium pentobarbital (ip), YZG-330 (0.125, 0.5 and 2 mg/kg, po) prolonged the sleep time by 25% (P>0.05), 64% (P<0.01) and 506% (P<0.001), respectively. Thereafter, treatment with YZG-330 permitted mice that had woken up after the threshold dose of sodium pentobarbital (ip) to fall asleep again. A Cl−-sensitive fluorescent probe, N-(ethoxycarbonylmethyl)-methoxyquinolinium bromide (MQAE), was used to determine the effect of YZG-330 on Cl− influx. YZG-330 (0.3, 0.6 and 1.5 mM) increased Cl− influx in mouse cortical cells in a concentration-dependent manner. These data suggest that YZG-330 has a hypnotic effect in mice, and the effect may be related to an increase in Cl− influx in cortical cells.

Keywords

• Sedative–hypnotic
• Sleep
• Cl− influx
• MQAE
• Cortical cells