High-throughput single-molecule quantification of individual base stacking energies in nucleic acids

Jibin Abraham Punnoose; Kevin J. Thomas; Arun Richard Chandrasekaran; Javier Vilcapoma; Andrew Hayden; Kacey Kilpatrick; Sweta Vangaveti; Alan Chen; Thomas Banco; Ken Halvorsen

doi:10.1038/s41467-023-36373-8

Nature Communications (Feb 2023)

High-throughput single-molecule quantification of individual base stacking energies in nucleic acids

Jibin Abraham Punnoose,
Kevin J. Thomas,
Arun Richard Chandrasekaran,
Javier Vilcapoma,
Andrew Hayden,
Kacey Kilpatrick,
Sweta Vangaveti,
Alan Chen,
Thomas Banco,
Ken Halvorsen

Affiliations

Jibin Abraham Punnoose: The RNA Institute, University at Albany, State University of New York
Kevin J. Thomas: The RNA Institute, University at Albany, State University of New York
Arun Richard Chandrasekaran: The RNA Institute, University at Albany, State University of New York
Javier Vilcapoma: The RNA Institute, University at Albany, State University of New York
Andrew Hayden: The RNA Institute, University at Albany, State University of New York
Kacey Kilpatrick: The RNA Institute, University at Albany, State University of New York
Sweta Vangaveti: The RNA Institute, University at Albany, State University of New York
Alan Chen: The RNA Institute, University at Albany, State University of New York
Thomas Banco: The RNA Institute, University at Albany, State University of New York
Ken Halvorsen: The RNA Institute, University at Albany, State University of New York

DOI: https://doi.org/10.1038/s41467-023-36373-8
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 13

Abstract

Read online

In this work, the authors use a centrifuge force microscope for high-throughput single-molecule experiments to elucidate stacking energies between individual bases of DNA.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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