Evaluation of Dynamic Thiol/Disulfide Homeostasis in Patients with Non-Muscle Invasive Bladder Tumor

Abstract

Objective:To evaluate the thiol-disulfide homeostasis in patients with a diagnosis of non-muscle invasive bladder tumor (NMIBC), which is a new oxidative stress marker, and to investigate the relationship between the development of NMIBC and native thiol, total thiol, and dynamic disulfide values.Materials and Methods:Fifty-three patients who were operated for bladder tumor in Karabük University Karabük Training and Research Hospital, Clinic of Urology between February and November 2020 and diagnosed with NMIBC in the pathological examination and 60 healthy volunteers were included in the study. Plasma native thiol, total thiol and disulfide levels of these two groups were measured and compared.Results:There was a statistically significant difference between the two groups in terms of native thiol, total thiol and disulfide values. In the subgroup analysis in those diagnosed with NMIBC, native thiol values were found to be 255,870 μmol/L in the low grade patient group and 169,420 μmol/L in the high grade patient group. This difference was statistically significant.Conclusion:The thiol disulfide homeostasis shifted to the disulfide side in the NMIBC group. It was determined that an increase in serum disulfide level and a decrease in native thiol level may have diagnostic value in predicting NMIBC. In addition, in the group diagnosed with NMIBC, there was a significant decrease in native thiol values as the pathological grade increased. This was interpreted as a shift of the equilibrium towards the oxidant side as the tumor showed an aggressive course.

Keywords

• bladder cancer
• thiol
• disulfide