eLife (Dec 2020)
Visualizing the metazoan proliferation-quiescence decision in vivo
- Rebecca C Adikes,
- Abraham Q Kohrman,
- Michael A Q Martinez,
- Nicholas J Palmisano,
- Jayson J Smith,
- Taylor N Medwig-Kinney,
- Mingwei Min,
- Maria D Sallee,
- Ononnah B Ahmed,
- Nuri Kim,
- Simeiyun Liu,
- Robert D Morabito,
- Nicholas Weeks,
- Qinyun Zhao,
- Wan Zhang,
- Jessica L Feldman,
- Michalis Barkoulas,
- Ariel M Pani,
- Sabrina L Spencer,
- Benjamin L Martin,
- David Q Matus
Affiliations
- Rebecca C Adikes
- ORCiD
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Abraham Q Kohrman
- ORCiD
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Michael A Q Martinez
- ORCiD
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Nicholas J Palmisano
- ORCiD
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Jayson J Smith
- ORCiD
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Taylor N Medwig-Kinney
- ORCiD
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Mingwei Min
- ORCiD
- Department of Biochemistry and BioFrontiers Institute, University of Colorado Boulder, Boulder, United States
- Maria D Sallee
- Department of Biology, Stanford University, Stanford, United States
- Ononnah B Ahmed
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Nuri Kim
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Simeiyun Liu
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Robert D Morabito
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Nicholas Weeks
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Qinyun Zhao
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Wan Zhang
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- Jessica L Feldman
- ORCiD
- Department of Biology, Stanford University, Stanford, United States
- Michalis Barkoulas
- ORCiD
- Department of Life Sciences, Imperial College, London, United Kingdom
- Ariel M Pani
- Department of Biology, University of Virginia, Charlottesville, United States
- Sabrina L Spencer
- ORCiD
- Department of Biochemistry and BioFrontiers Institute, University of Colorado Boulder, Boulder, United States
- Benjamin L Martin
- ORCiD
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- David Q Matus
- ORCiD
- Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United States
- DOI
- https://doi.org/10.7554/eLife.63265
- Journal volume & issue
-
Vol. 9
Abstract
Cell proliferation and quiescence are intimately coordinated during metazoan development. Here, we adapt a cyclin-dependent kinase (CDK) sensor to uncouple these key events of the cell cycle in Caenorhabditis elegans and zebrafish through live-cell imaging. The CDK sensor consists of a fluorescently tagged CDK substrate that steadily translocates from the nucleus to the cytoplasm in response to increasing CDK activity and consequent sensor phosphorylation. We show that the CDK sensor can distinguish cycling cells in G1 from quiescent cells in G0, revealing a possible commitment point and a cryptic stochasticity in an otherwise invariant C. elegans cell lineage. Finally, we derive a predictive model of future proliferation behavior in C. elegans based on a snapshot of CDK activity in newly born cells. Thus, we introduce a live-cell imaging tool to facilitate in vivo studies of cell-cycle control in a wide-range of developmental contexts.
Keywords
