Um fotômetro de fluxo para análises clínicas a base de um diodo emissor de luz bicolor An inexpensive flow photometer for clinical analysis based on a bicolour light emitting diode

Abstract

The construction and evaluation of an inexpensive flow photometer for clinical analysis, using a bicolour LED and a phototransistor adapted for tubular flow cell, are described. The instrument presents some new features such as: automatic zero, electronic calibration and peak-hold signal. When compared with a classical photometer, it is simpler and has the advantages of a flow analysis system: lower volumes of reagents and samples, lower levels of contamination, shorter time for analysis and lower analysis costs. The instrument was used in the determination of the constituents in blood samples. The results obtained agree with those obtained by a classical photometer and the precision was better.

Keywords

• flow photometer
• light emitting diode
• clinical analysis