Characterization of Nitrogen-Fixing Cyanobacterial Consortia Isolated from the Rhizosphere of <i>Carica papaya</i>
Yadenis Ortega Díaz,
Liliana Gomez Luna,
Yilan Fung Boix,
Yadira Silveira Font,
Jorge González Aguilera,
Obert Marín-Sánchez,
Abel Walter Zambrano-Cabanillas,
Olegario Marín-Machuca,
Sheda Méndez-Ancca,
Carlos Enrique Chinchay Barragán
Affiliations
Yadenis Ortega Díaz
National Centre of Applied Electromagnetism, University of Oriente, Santiago de Cuba 90600, Cuba
Liliana Gomez Luna
National Centre of Applied Electromagnetism, University of Oriente, Santiago de Cuba 90600, Cuba
Yilan Fung Boix
National Centre of Applied Electromagnetism, University of Oriente, Santiago de Cuba 90600, Cuba
Yadira Silveira Font
National Centre of Applied Electromagnetism, University of Oriente, Santiago de Cuba 90600, Cuba
Jorge González Aguilera
Department of Crop Science, State University of Mato Grosso do Sul, Cassilândia 79540-000, Brazil
Obert Marín-Sánchez
Academic Department of Medical Microbiology, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru
Abel Walter Zambrano-Cabanillas
Academic Department of Aquaculture, Faculty of Oceanography, Fisheries, Food Science and Aquaculture, Universidad Nacional Federico Villarreal, Lima 15001, Peru
Olegario Marín-Machuca
Professional School of Food Engineering, Faculty of Oceanography, Fisheries, Food Sciences and Aquaculture (FOPCA), Environmental Sustainability Research Group (GISA), University Graduate School (EUPG), Federico Villarreal National University, Lima 15088, Peru
Sheda Méndez-Ancca
Academic Department of Pesquera Engineering, National University of Moquegua (UNAM), Ilo 18601, Peru
Carlos Enrique Chinchay Barragán
Escuela Profesional de Ingeniería de Alimentos, Facultad de Ingeniería Pesquera y de Alimentos, Universidad Nacional del Callao, Callao 07011, Peru
Nitrogen-fixing cyanobacterial consortia are an alternative to the indiscriminate use of chemical fertilizers that affect the environment, fix atmospheric nitrogen, and can therefore be used as plant growth promoters, synthesize various substances such as auxins, vitamins, and total proteins, and fix atmospheric biofertilizers and soil conditioners. The present study aimed to obtain and develop, by biotechnological means, two consortia of cyanobacteria isolated from the root and rhizosphere of Carica papaya grown in sandy loam soil. The culture was carried out in Blue Green Medium without modified nitrogen (BG110), with aeration of 0.32 L min−1, at a light intensity of 48.83 μEm−2s−1, and a temperature of 22 ± 2 °C. Two consortia consisting of Nostoc commune, Aphanothece minutissima, Planktothrix sp. (C1), Nostoc commune, Calothrix sp., and Aphanothece minutissima (C2) were isolated and morphologically identified. The effective development of these consortia was verified at the laboratory level by obtaining biomass in dry weight as well as photosynthetic pigments, proteins, carbohydrates, and lipids. Germination parameters were determined in seeds of Cucumis sativus L. var. Market plus treated with the nitrogen-fixing cyanobacteria consortia, obtaining a higher germination percentage (>90%), greater root length (>6 cm), and higher vigour index I (513), II (13.02) for the C2 consortium. This broadens the spectrum of rhizosphere-derived microorganisms with potential as growth biostimulators.