Oxidation of F-actin controls the terminal steps of cytokinesis

Stéphane Frémont; Hussein Hammich; Jian Bai; Hugo Wioland; Kerstin Klinkert; Murielle Rocancourt; Carlos Kikuti; David Stroebel; Guillaume Romet-Lemonne; Olena Pylypenko; Anne Houdusse; Arnaud Echard

doi:10.1038/ncomms14528

Nature Communications (Feb 2017)

Oxidation of F-actin controls the terminal steps of cytokinesis

Stéphane Frémont,
Hussein Hammich,
Jian Bai,
Hugo Wioland,
Kerstin Klinkert,
Murielle Rocancourt,
Carlos Kikuti,
David Stroebel,
Guillaume Romet-Lemonne,
Olena Pylypenko,
Anne Houdusse,
Arnaud Echard

Affiliations

Stéphane Frémont: Membrane Traffic and Cell Division Lab, Cell Biology and Infection Department Institut Pasteur
Hussein Hammich: Structural Motility, Institut Curie, PSL Research University,
Jian Bai: Membrane Traffic and Cell Division Lab, Cell Biology and Infection Department Institut Pasteur
Hugo Wioland: Institut Jacques Monod, CNRS, Université Paris Diderot, Université Sorbonne Paris Cité
Kerstin Klinkert: Membrane Traffic and Cell Division Lab, Cell Biology and Infection Department Institut Pasteur
Murielle Rocancourt: Membrane Traffic and Cell Division Lab, Cell Biology and Infection Department Institut Pasteur
Carlos Kikuti: Structural Motility, Institut Curie, PSL Research University,
David Stroebel: Ecole Normale Supérieure, PSL Research University, CNRS, INSERM, Institut de Biologie de l'École Normale Supérieure (IBENS)
Guillaume Romet-Lemonne: Institut Jacques Monod, CNRS, Université Paris Diderot, Université Sorbonne Paris Cité
Olena Pylypenko: Structural Motility, Institut Curie, PSL Research University,
Anne Houdusse: Structural Motility, Institut Curie, PSL Research University,
Arnaud Echard: Membrane Traffic and Cell Division Lab, Cell Biology and Infection Department Institut Pasteur

DOI: https://doi.org/10.1038/ncomms14528
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 16

Abstract

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Cytokinetic abscission relies on the local constriction after cytoskeleton disassembly, but it is not known how the actin filaments are disassembled. Here, the authors show that the redox enzyme MICAL1 is recruited by Rab35 and induces oxidation-mediated depolymerization of actin, which is required to recruit ESCRT-III and complete abscission.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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