HT-29 Colon Cancer Cell Electromanipulation and Assessment Based on Their Electrical Properties
Marius Andrei Olariu,
Catalin Tucureanu,
Tudor Alexandru Filip,
Iuliana Caras,
Aurora Salageanu,
Valentin Vasile,
Marioara Avram,
Bianca Tincu,
Ina Turcan
Affiliations
Marius Andrei Olariu
Department of Electrical Measurements and Materials, Faculty of Electrical Engineering and Information Technology, Gheorghe Asachi Technical University of Iasi, 700050 Iasi, Romania
Catalin Tucureanu
Cantacuzino, National Medical-Military Institute for Research and Development, 050096 Bucharest, Romania
Tudor Alexandru Filip
Department of Electrical Measurements and Materials, Faculty of Electrical Engineering and Information Technology, Gheorghe Asachi Technical University of Iasi, 700050 Iasi, Romania
Iuliana Caras
Cantacuzino, National Medical-Military Institute for Research and Development, 050096 Bucharest, Romania
Aurora Salageanu
Cantacuzino, National Medical-Military Institute for Research and Development, 050096 Bucharest, Romania
Valentin Vasile
Cantacuzino, National Medical-Military Institute for Research and Development, 050096 Bucharest, Romania
Marioara Avram
National Institute for Research and Development in Microtechnologies-IMT Bucharest, 077190 Bucharest, Romania
Bianca Tincu
National Institute for Research and Development in Microtechnologies-IMT Bucharest, 077190 Bucharest, Romania
Ina Turcan
Department of Electrical Measurements and Materials, Faculty of Electrical Engineering and Information Technology, Gheorghe Asachi Technical University of Iasi, 700050 Iasi, Romania
This study proposes a feasible approach for the rapid, sensitive, and label-free identification of cancerous cells based on dielectrophoretic (DEP) manipulation and electrical characterization. In this method, the concentration of target cells at the level of customized microelectrodes via DEP is first determined, followed by an electrical impedance evaluation. The study demonstrates the capacity of the methodology to electrically differentiate HT-29 cancer cells from healthy blood cells based on their impedance spectra. Within a higher frequency domain, the electrical impedance of trapped cancer cells was significantly lower compared with the normal ones. In order to evaluate the functionality and reproducibility of the proposed method, the influence of the DEP and EIS (electrical impedance spectroscopy) operating voltages on the electrical characterization of trapped HT-29 cells was analyzed.
