Sequence-Based Identification of Metronidazole-Resistant Clostridioides difficile Isolates

Abstract

The plasmid pCD-METRO confers metronidazole resistance in Clostridioides difficile. We showed high sequence similarity among pCD-METRO plasmids from different isolates and identified pCD-METRO and associated metronidazole-resistant isolates in clinical and veterinary reservoirs in the Americas. We recommend using PCR or genomic assays to detect pCD-METRO in metronidazole-resistant C. difficile.

Keywords

• Clostridioides difficile
• Antimicrobial resistance
• bacteria
• enteric infections
• metronidazole
• plasmid