A mesenchymal stem cell-derived nanovesicle-biopotentiated bovine serum albumin-bridged gelatin hydrogel for enhanced diabetic wound therapy

Yan Wu; Meng Su; Shihao Zhang; Lan Xiao; Yin Xiao; Mengya Zhang; Ying Bei; Meiyun Li; Fan Zhang; Qi Yuan; Shiyong Wu; Jianyong Liu; Haitao Li; Xiaohuan Yuan; Meng Li; Yulin Li; Jie Gao

Materials & Design (Jun 2023)

A mesenchymal stem cell-derived nanovesicle-biopotentiated bovine serum albumin-bridged gelatin hydrogel for enhanced diabetic wound therapy

Yan Wu,
Meng Su,
Shihao Zhang,
Lan Xiao,
Yin Xiao,
Mengya Zhang,
Ying Bei,
Meiyun Li,
Fan Zhang,
Qi Yuan,
Shiyong Wu,
Jianyong Liu,
Haitao Li,
Xiaohuan Yuan,
Meng Li,
Yulin Li,
Jie Gao

Affiliations

Yan Wu: College of Life Science, Mudanjiang Medical University, Mudanjiang, China
Meng Su: College of Life Science, Mudanjiang Medical University, Mudanjiang, China
Shihao Zhang: The Key Laboratory for Ultrafine Materials of Ministry of Education, State Key Laboratory of Bioreactor Engineering, Engineering Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, China
Lan Xiao: School of Mechanical, Medical and Process Engineering, Center of Biomedical Technology, Queensland University of Technology, Brisbane, Australia; Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, Australia
Yin Xiao: School of Mechanical, Medical and Process Engineering, Center of Biomedical Technology, Queensland University of Technology, Brisbane, Australia; Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, Australia; School of Medicine and Dentistry, Griffith University, Queensland, Australia
Mengya Zhang: Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai, China
Ying Bei: College of Life Science, Mudanjiang Medical University, Mudanjiang, China
Meiyun Li: College of Life Science, Mudanjiang Medical University, Mudanjiang, China
Fan Zhang: College of Life Science, Mudanjiang Medical University, Mudanjiang, China
Qi Yuan: College of Life Science, Mudanjiang Medical University, Mudanjiang, China
Shiyong Wu: The Key Laboratory for Ultrafine Materials of Ministry of Education, State Key Laboratory of Bioreactor Engineering, Engineering Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, China
Jianyong Liu: Department of Vascular Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Haitao Li: Department of Vascular Surgery, Wuhan Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
Xiaohuan Yuan: College of Life Science, Mudanjiang Medical University, Mudanjiang, China; Corresponding authors at: Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China (J. Gao). The Key Laboratory for Ultrafine Materials of Ministry of Education, State Key Laboratory of Bioreactor Engineering, Engineering Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China (Y. Li). Department of Dermatology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University, Shanghai 200010, China (M. Li). College of Life Science, Mudanjiang Medical University, Mudanjiang 157011, China (X. Yuan).
Meng Li: Department of Dermatology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University, Shanghai, China; Corresponding authors at: Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China (J. Gao). The Key Laboratory for Ultrafine Materials of Ministry of Education, State Key Laboratory of Bioreactor Engineering, Engineering Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China (Y. Li). Department of Dermatology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University, Shanghai 200010, China (M. Li). College of Life Science, Mudanjiang Medical University, Mudanjiang 157011, China (X. Yuan).
Yulin Li: The Key Laboratory for Ultrafine Materials of Ministry of Education, State Key Laboratory of Bioreactor Engineering, Engineering Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, China; Corresponding authors at: Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China (J. Gao). The Key Laboratory for Ultrafine Materials of Ministry of Education, State Key Laboratory of Bioreactor Engineering, Engineering Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China (Y. Li). Department of Dermatology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University, Shanghai 200010, China (M. Li). College of Life Science, Mudanjiang Medical University, Mudanjiang 157011, China (X. Yuan).
Jie Gao: Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai, China; Corresponding authors at: Changhai Clinical Research Unit, Shanghai Changhai Hospital, Naval Medical University, Shanghai 200433, China (J. Gao). The Key Laboratory for Ultrafine Materials of Ministry of Education, State Key Laboratory of Bioreactor Engineering, Engineering Research Center for Biomedical Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China (Y. Li). Department of Dermatology, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University, Shanghai 200010, China (M. Li). College of Life Science, Mudanjiang Medical University, Mudanjiang 157011, China (X. Yuan).

Journal volume & issue: Vol. 230
p. 111960

Abstract

Read online

The application of gelatin hydrogels is hampered by their poor mechanical properties, which are unfavorable for debridement and diabetic wound healing. Herein, bovine serum albumin (BSA)-bridged gelatin hydrogels biopotentiated by bone marrow mesenchymal stem cell (BMSC)-derived nanovesicles (NV), named NV@BSA-GEL, were fabricated by reinforcement of gelatin hydrogels with genipin and BSA in a one-pot, low-cost green approach based on amide bonding-guided crosslinking. Genipin introduction endowed the crosslinking of gelatin hydrogels with weak mechanical properties that were reinforced by BSA bridging to realize suitable mechanical properties for wound dressing, including excellent flexibility and appropriate rigidity. The bioactivity of NV released by the hydrogels resulted in efficient ROS scavenging and favorable immunomodulatory activity as well as promoted cell proliferation and migration of fibroblasts and vascular endothelial cells. Proteomic analysis confirmed that NV harbors a variety of growth factors that can promote wound healing. In vivo, the NV@BSA-GEL hydrogel had superior wound healing effects compared to BSA-GEL, including faster wound healing, stimulation of angiogenesis, contributing to an anti-inflammatory extracellular matrix, and promoting skin maturation, with no systemic toxicity. Thus, the combined merits of NV@BSA-GEL suggest that it has high potential as a new hydrogel dressing for the safe and efficient treatment of diabetic wounds.

Published in Materials & Design

ISSN: 0264-1275 (Print); 1873-4197 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://www.journals.elsevier.com/materials-and-design

About the journal

Abstract

Keywords