MULTISPECIES BIOFILM FORMATION BY LACTIC ACID BACTERIA LACTOBACILLUS PLANTARUM Р17630 AND YEASTS САNDIDА АLBIСАNS АТСС 18804

Abstract

Aim. The study Lactobacillus plantarum Р17630 and Candida albisans АТСС 18804 cell interaction in multispecies biofilm and its main formation stage identification. Methods. Interaction of Lactobacillus рlantarum Р17630 and Саndidа аlbiсапs АТСС 18804 cells during the formation of mono- and multispecies biofilm was studied using culture media: MRS and Sabouraud. The biofilm structure and certain structural component presence determined the maturity level of microbial associations were detected by crystal violet staining. The interactions were registered during the polyspecies biofilm formation by comparing its optical density with L. plantarum Р17630 and C. albicans АТСС 18804 monospecies ones. Results. The most intensive formation of monospecies biofilms by individual L. рlantarum Р17630 and С. аlbiсапs ATCC 18804 strains occurred within the first two days cultivation in liquid medium Sabouraud. The most manifestation of the developed antagonistic interaction between L. рlantarum and С. аlbiсапs was observed for the first 48 hrs of cultivation, defining breach of the structural biofilm components. Conclusions. The adhesion of cells to solid surface (first 24 hrs), the individual microcolony for mation (for the second day) and mature cell layer forming (96 hrs incubation) were three main phases during the monospecies biofilm formation. The antagonistic interaction between L. рlantarum and С. аlbiсапs as a part of multispecies biofilms, in particular slowing down the yeast development and mature cell layer formation, was more influenced on the hyphae community generation than individual yeast cells.

Keywords

• полівидова біоплівка
• lactobacillus plantarum
• candida albicans
• антагонізм