Journal of Applied Animal Research (Dec 2023)

Selected hematological parameters and oxidative stress in red blood cells (RBCs) after accidental cases of nitrate toxicity in the goat fed cabbage

  • Atef M. Khalil,
  • Ibrahim F. Rehan,
  • František Zigo,
  • Šimon Halás,
  • Zuzana Farkašová,
  • Hassan M. Diab

DOI
https://doi.org/10.1080/09712119.2023.2194360
Journal volume & issue
Vol. 51, no. 1
pp. 300 – 307

Abstract

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Nitrate poisoning due to the consumption of cabbages was diagnosed in a small goat flock, in Qena city, Egypt. The methylene blue 1% antidote (1 mg/kg BW) was given intravenously to the poisoned goats. Nitrate poisoning was confirmed in blood plasma, saliva, urine, and cabbage dry matter samples. The dark brown blood and methemoglobin fraction were 47.25 ± 0.58%. Green cabbage containing 7.1% nitrate on dried materials was fed to the goats. The nitrate concentration in the saliva was (59.50 ± 4.67), compared to the healthy goats reared in the same area and tested positive in the plasma and urine of intoxicated goats. Intoxication induced a significant decline in the Red Blood Cells count, hemoglobin concentration, and HCT % (P < 0.001), while the WBC count (P < 0.001), neutrophils (P < 0.001), and monocyte percentage were elevated (P < 0.002) in intoxicated goats, while the lymphocyte % (P < 0.01) significantly decreased when compared to the control. For the oxidative stress in the red blood cells, nitrate toxicity caused a significant elevation in the concentration of Malondialdehyde, (P < 0.001) while Superoxide Dismutase, Glutathione, and Glutathione S-Transferase levels were significantly reduced (P < 0.001) compared to the control. In this current report, the ingestion of goat to cabbage containing a high level of nitrate exhibited nitrate-nitrite intoxication.

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