Genel Tıp Dergisi (Feb 2024)
Evaluation of In Vitro Antimicrobial Activity of Epigallocatechin Gallate (EGCG) and Green Tea (Camellia Sinensis) Oil on Various Pathogens
Abstract
Aim: EGCG, the polyphenolic component of Camellia sinesis catechins, and Camellia sinesis extract have broad antimicrobial activity. To investigate the invitro antibacterial and antifungal activity of the Epigallocatechin gallate and Camellia sinesis extract. Method: The present study tested the antibacterial and antifungal activity of Epigallocatechin gallate and Camellia sinesis extract against some gram-negative, gram-positive, and fungal isolates of American Type Culture Collection (ATCC). The minimum inhibitory concentration (MIC) of Epigallocatechin gallate (EGCG) and Camellia sinensis extract were determined for each test microorganism. In general, EGCG and Camellia sinensis extract results were found to be compatible. Epigallocatechin gallate and Camellia sinesis extract exhibited antibacterial and antifungal activity against all test organisms. MIC was determined visually after 16-20 hours of incubation at 37°C according to broth microdilution methods recommended by the Clinical and Laboratory Standards Institute (CLSI). Result: The Camellia sinesis extract and EGCG MIC for gram-positive bacteria was found to be lower than the MIC for gram-negative bacteria and the Camellia sinesis extract MIC for C. albicans was found to be higher than the EGCG MIC. Conclusions: However, EGCG was found to be more effective on C. albicans isolate than Camellia sinensis extract. Camellia sinesis extract Epigallocatechin gallate and Camellia sinesis extract may be a promising sources of antibacterial and antifungal for further studies.
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