Transcriptomic data from the rat liver after five days of exposure to legacy or emerging brominated flame retardants
Keith R. Shockley,
Michelle C. Cora,
David E. Malarkey,
Daven Jackson-Humbles,
Molly Vallant,
Brad J. Collins,
Esra Mutlu,
Veronica G. Robinson,
Surayma Waidyanatha,
Amy Zmarowski,
Nicholas Machesky,
Jamie Richey,
Sam Harbo,
Emily Cheng,
Kristin Patton,
Barney Sparrow,
June K. Dunnick
Affiliations
Keith R. Shockley
Biostatistics & Computational Biology Branch, Division of Intramural Research, National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC 27709, United States; Corresponding author.
Michelle C. Cora
Cellular & Molecular Pathology Branch, Division of the National Toxicology Program (DNTP), NIEHS, Research Triangle Park, NC 27709, United States
David E. Malarkey
Cellular & Molecular Pathology Branch, Division of the National Toxicology Program (DNTP), NIEHS, Research Triangle Park, NC 27709, United States
Daven Jackson-Humbles
Cellular & Molecular Pathology Branch, Division of the National Toxicology Program (DNTP), NIEHS, Research Triangle Park, NC 27709, United States
Molly Vallant
Program Operations Branch, DNTP, NIEHS, Research Triangle Park, NC 27709, United States
Brad J. Collins
Program Operations Branch, DNTP, NIEHS, Research Triangle Park, NC 27709, United States
Esra Mutlu
Program Operations Branch, DNTP, NIEHS, Research Triangle Park, NC 27709, United States
Veronica G. Robinson
Program Operations Branch, DNTP, NIEHS, Research Triangle Park, NC 27709, United States
Surayma Waidyanatha
Program Operations Branch, DNTP, NIEHS, Research Triangle Park, NC 27709, United States
Amy Zmarowski
Battelle, Columbus, Ohio 43210, United States
Nicholas Machesky
Battelle, Columbus, Ohio 43210, United States
Jamie Richey
Battelle, Columbus, Ohio 43210, United States
Sam Harbo
Battelle, Columbus, Ohio 43210, United States
Emily Cheng
Battelle, Columbus, Ohio 43210, United States
Kristin Patton
Battelle, Columbus, Ohio 43210, United States
Barney Sparrow
Battelle, Columbus, Ohio 43210, United States
June K. Dunnick
Toxicology Branch, DNTP, NIEHS, Research Triangle Park, NC 27709, United States
Large-scale gene expression analysis of legacy* and emerging** brominated flame retardants were conducted in the male Harlan Sprague Dawley rat [1]. Each animal was dosed for 5 days with the chemical at concentrations of 0.1 – 1000 μmol/kg body weight per day. Following the last dose, a specimen of the left liver was removed for RNA extraction. The amplified RNA (aRNA) was fragmented and then hybridized to Affymetrix Rat Genome 230 2.0 Arrays. Each GeneChip® array was scanned using an Affymetrix GeneChip® Scanner 3000 7 G to generate raw expression level data (.CEL files). Statistical contrasts were used to find pairwise gene expression differences between the control group and each dose group using the R/maanova package [2]. The transcriptomic data can be used to provide insights into the degree of toxicity, toxic mechanisms, disease pathways activated by exposure, and for benchmark dose analysis. The gene expression data for each of the nine flame retardants discussed here accompanies the research article entitled, “Comparative Toxicity and Liver Transcriptomics of Legacy and Emerging Brominated Flame Retardants following 5-Day Exposure in the Rat” [1].* polybrominated diphenyl ether 47 (PBDE 47), decabromodiphenyl ether (decaBDE), hexabromocyclododecane (HBCD); ** 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB); bis(2-ethylhexyl) tetrabromophthalate (TBPH); tetrabromobisphenol A-bis(2,3-dibromopropyl ether (TBBPA-DBPE); 1,2-bis(tribromophenoxy)ethane (BTBPE); decabromodiphenylethane (DBDPE); hexachlorocyclopentadienyl-dibromocyclooctane (HCDBCO).
