Quantitative tRNA-sequencing uncovers metazoan tissue-specific tRNA regulation

Otis Pinkard; Sean McFarland; Thomas Sweet; Jeff Coller

doi:10.1038/s41467-020-17879-x

Nature Communications (Aug 2020)

Quantitative tRNA-sequencing uncovers metazoan tissue-specific tRNA regulation

Otis Pinkard,
Sean McFarland,
Thomas Sweet,
Jeff Coller

Affiliations

Otis Pinkard: Department of Genetics and Genome Sciences, Case Western Reserve University
Sean McFarland: Tevard Biosciences, LabCentral
Thomas Sweet: Department of Nutrition, Case Western Reserve University
Jeff Coller: Department of Molecular Biology & Genetics and Department of Biology, Johns Hopkins School of Medicine

DOI: https://doi.org/10.1038/s41467-020-17879-x
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 15

Abstract

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The relative abundance of specific tRNA can impact protein production rate, folding, and messenger RNA stability. Here the authors describe QuantM-tRNA seq — a method to monitor tRNA abundance and sequence variants — and uncover distinctions in isodecoder expression between tissues that are independent of the anticodon pool of each tRNA family.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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