Analysis of the metabolic properties of maintenance hemodialysis patients with glucose-added dialysis based on high performance liquid chromatography quadrupole time-of-flight mass spectrometry

Abstract

Li Cui,1 Yu Meng,2 Dan Xu,2 Yanyan Feng,2 Gangyi Chen,3 Bo Hu,2 Guijuan Feng,4 Lianghong Yin2 1Xi'xiang People's Hospital Affiliated to Guangdong Medical College, Shenzhen, People's Republic of China; 2Nephrology Department of the First Hospital Affiliated to Ji'nan University, Guangzhou, People's Republic of China; 3First Hospital of Guangzhou University of Chinese Medicine, 4Assisted Reproductive Centre of the First Hospital Affiliated to Ji'nan University, Guangzhou, People's Republic of China Abstract: The purpose of this study was to compare the metabolic properties of maintenance hemodialysis patients treated with glucose-containing and glucose-free dialysate using metabonomics. Pre- and post-dialysis serum samples from group G (−) using glucose-free dialysate, and group G (+) using glucose-added dialysate (glucose levels were 5.5 mmol/L) were analyzed and tested with high performance liquid chromatography quadrupole time-of-flight mass spectrometry. Orthogonal signal correction–partial least squares discriminate analysis revealed a significant difference in the post-dialysis metabolic properties between samples from the G (−) and G (+) groups, and concentrations of leucine and dihydroxyprostaglandin F2α were higher in the G (+) group than in the G (−) group. However, markers of reactive lipid mobilization and amino acid release, such as bile acids, aspartate, and valine, were lower in the G (+) group than in the G (−) group. There were no significant differences in excitatory neurotransmitters aspartate and phosphorylated anandamide. Use of liquid chromatography-tandem mass spectrometry metabonomics indicated that using glucose-added dialysate was superior to glucose-free dialysate in the protection of the central nervous system of maintenance hemodialysis patients, but had potential risks in stimulating oxidative stress. Keywords: hemodialysis, metabonomics, glucose-added dialysate