Sublethal doses of diazinon affected reproductive, immune, and oxidative status in female zebrafish (Danio rerio)

Abstract

Diazion is a common pesticide which cultured fish can be exposed. There is limited information available on the possible effects of diazinon on reproductive parameters of fish. Therefore, in this study, we focused on the effects of sub-lethal doses (0, 0.8, 1.6, and 3.2 mg/L) of diazinon, an organophosphate insecticide on sex hormones, ovarian morphology, reproductive, immunity, and antioxidant related genes, in female zebrafish (Danio rerio) after 30 days of treatment. The expression of reproductive-related genes (CYP19a, Vtg, and ERα) significantly decreased, while the expression of the immune (TNFα and Il-1β) and antioxidant (SOD and CAT) related genes increased considerably. Among sex steroids, only progesterone significantly decreased after the treatment with Diazinon sub-lethal doses, while 17-β estradiol and testosterone did not change. The ovarian morphology showed a decrease in oocyte development in diazinon-treated groups. Understanding the function and mechanism of action of diazinon on the female reproductive, immune, and oxidant status may provide new strategies to counteract its adverse effects.

Keywords

• Diazinon
• Zebrafish
• Sex hormones
• Ovary
• Vitellogenin
• Estrogen receptor