A novel fluorescent probe for the detection of peroxynitrite and its application in acute liver injury model
Chen Jin,
Pengfei Wu,
Yushun Yang,
Zhenxiang He,
Hailiang Zhu,
Zhen Li
Affiliations
Chen Jin
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing, 210023, China
Pengfei Wu
Pancreas Center, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, Nanjing, 210029, China; Pancreas Institute of Nanjing Medical University, Nanjing, 210029, China
Yushun Yang
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing, 210023, China
Zhenxiang He
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing, 210023, China; Corresponding author.
Hailiang Zhu
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing, 210023, China; Corresponding author.
Zhen Li
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing, 210023, China; Corresponding author.
Realizing the early diagnosis of acute liver injury has become a hotspot in recent years. Since the current indexes are not specific, developing novel probes with new recognition group remains a necessity. In this work, we developed a novel fluorescent probe, NNP, for the detection of ONOO− in acute liver injury (ALI). The in vitro evaluation of NNP indicated the advantages including high sensitivity (LOD = 0.13 μM), rapid response (48 h) conditions. Accordingly, NNP achieved the dose-dependent detection of ONOO− in human hepatic stellate LX-2 cells. Further, in the ALI model mice, NNP could monitor the ONOO− level in the occurrence of ALI in situ (in the liver region) with a steady performance. Subsequent immunohistochemical and imaging studies confirmed that NNP could achieve the detection of endogenous ONOO− in ALI liver tissues. This work introduced a practical implement for the ONOO− detection in ALI as well as a referable example for the establishment of molecular indexes in pre-clinical diagnosis.
