Nature Communications (Jan 2020)

Generation of mesenchyme free intestinal organoids from human induced pluripotent stem cells

  • Aditya Mithal,
  • Amalia Capilla,
  • Dar Heinze,
  • Andrew Berical,
  • Carlos Villacorta-Martin,
  • Marall Vedaie,
  • Anjali Jacob,
  • Kristine Abo,
  • Aleksander Szymaniak,
  • Megan Peasley,
  • Alexander Stuffer,
  • John Mahoney,
  • Darrell N. Kotton,
  • Finn Hawkins,
  • Gustavo Mostoslavsky

DOI
https://doi.org/10.1038/s41467-019-13916-6
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 15

Abstract

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Human induced pluripotent stem cell-derived intestinal organoids (HIOs) are powerful tools to study development and diseases of the gastrointestinal tract. Here, the authors develop a directed differentiation protocol to generate mesenchyme-free HIOs that can be patterned towards proximal small intestine or colonic epithelium, and demonstrated their utility in modeling CFTR function.