Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia
Michaela Mrkvová,
Jana Kemenczeiová,
Adam Achs,
Peter Alaxin,
Lukáš Predajňa,
Katarína Šoltys,
Zdeno Šubr,
Miroslav Glasa
Affiliations
Michaela Mrkvová
Department of Biology, Faculty of Natural Sciences, Institute of Biology and Biotechnology, University of Ss. Cyril and Methodius in Trnava, Námestie J. Herdu 2, 917 01 Trnava, Slovakia
Jana Kemenczeiová
Department of Biology, Faculty of Natural Sciences, Institute of Biology and Biotechnology, University of Ss. Cyril and Methodius in Trnava, Námestie J. Herdu 2, 917 01 Trnava, Slovakia
Adam Achs
Institute of Virology, Biomedical Research Center of Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovakia
Peter Alaxin
Department of Biology, Faculty of Natural Sciences, Institute of Biology and Biotechnology, University of Ss. Cyril and Methodius in Trnava, Námestie J. Herdu 2, 917 01 Trnava, Slovakia
Lukáš Predajňa
Institute of Virology, Biomedical Research Center of Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovakia
Katarína Šoltys
Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, 842 15 Bratislava, Slovakia
Zdeno Šubr
Institute of Virology, Biomedical Research Center of Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovakia
Miroslav Glasa
Department of Biology, Faculty of Natural Sciences, Institute of Biology and Biotechnology, University of Ss. Cyril and Methodius in Trnava, Námestie J. Herdu 2, 917 01 Trnava, Slovakia
Analysis of the viromes of three symptomatic Fabaceae plants, i.e., red clover (Trifolium pratense L.), pea (Pisum sativum L.), and common bean (Phaseolus vulgaris L.), using high-throughput sequencing revealed complex infections and enabled the acquisition of complete genomes of a potyvirus, bean yellow mosaic virus (BYMV). Based on phylogenetic analysis, the Slovak BYMV isolates belong to two distinct molecular groups, i.e., VI (isolate FA40) and XI (isolates DAT, PS2). Five commercial pea genotypes (Alderman, Ambrosia, Gloriosa, Herkules, Senator) were successfully infected with the BYMV-PS2 inoculum and displayed similar systemic chlorotic mottling symptoms. Relative comparison of optical density values using semi-quantitative DAS-ELISA revealed significant differences among virus titers in one of the infected pea genotypes (Ambrosia) when upper fully developed leaves were tested. Immunoblot analysis of systemically infected Alderman plants showed rather uneven virus accumulation in different plant parts. The lowest virus accumulation was repeatedly detected in the roots, while the highest was in the upper part of the plant stem.
