Effects of Long-Term Coated Sodium Butyrate Supplementation on the Intestinal Health and Colonization of Cecal <i>Salmonella</i> of Laying Hens Infected with <i>Salmonella enteritidis</i>
Siyu Xiong,
Qi Zhang,
Keying Zhang,
Jianping Wang,
Shiping Bai,
Qiufeng Zeng,
Huanwei Peng,
Yue Xuan,
Yadong Mu,
Xuemei Ding
Affiliations
Siyu Xiong
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Qi Zhang
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Keying Zhang
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Jianping Wang
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Shiping Bai
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Qiufeng Zeng
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Huanwei Peng
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Yue Xuan
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Yadong Mu
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Xuemei Ding
Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, 211 Huimin Road, Wenjiang District, Chengdu 611130, China
Salmonella enterica ser. Enteritidis (S. Enteritidis) is widely found in chickens and eggs, and it can potentially induce human illness. The investigation in this study centers on the impacts of long-term dietary supplementation with coated sodium butyrate (CSB) on intestinal well-being and the colonization of cecum Salmonella in laying hens infected with S. Enteritidis. We segregated a total of 120 Lohmann laying hens aged 51 weeks into four treatment categories: 0 (CON), 300 (CSB1), 500 (CSB2), and 800 (CSB3) mg/kg of CSB, supplemented with CSB from the first day of the experiment. A 24-week observation process was carried out for each laying hen. The S. Enteritidis was orally administered to all chickens on the morning of the first and third days of week 22 of the trial. After the S. Enteritidis challenge, egg production decreased the most in the CON group. Compared to the CON group, the three doses of CSB significantly improved egg production after the S. Enteritidis challenge (PANOVA S. Enteritidis challenge increased plasma DAO activity, but CSB supplementation reduced plasma DAO activity (Plinear S. Enteritidis challenge disrupted intestinal villi morphology; compared to the CON group, the three dosages of CSB resulted in an increase in villus height (VH) and the ratio of villus height to crypt depth (V/C) in the duodenum, jejunum, and ileum of infected laying hens (Plinear PANOVA Plinear PANOVA Plinear IL-6 and IL-1β in jejunum (Plinear IL-1β in ileum (PANOVA IL-10 in ileum (Plinear ZO-1 and CLDN1 (PANOVA Salmonella and increased the number of Lactobacilli in the cecum (Plinear Plinear = 0.069) and reduce the E. coli count (Plinear = 0.081). In conclusion, long-term dietary supplementation with coated sodium butyrate can alleviate intestinal injury and the colonization of cecum Salmonella in laying hens infected with S. Enteritidis.
