Frontiers in Microbiology (May 2017)

Helicobacter pylori Antibody Reactivities and Colorectal Cancer Risk in a Case-control Study in Spain

  • Nerea Fernández de Larrea-Baz,
  • Nerea Fernández de Larrea-Baz,
  • Angelika Michel,
  • Beatriz Romero,
  • Beatriz Pérez-Gómez,
  • Beatriz Pérez-Gómez,
  • Beatriz Pérez-Gómez,
  • Victor Moreno,
  • Victor Moreno,
  • Victor Moreno,
  • Victor Moreno,
  • Vicente Martín,
  • Vicente Martín,
  • Vicente Martín,
  • Trinidad Dierssen-Sotos,
  • Trinidad Dierssen-Sotos,
  • José J. Jiménez-Moleón,
  • José J. Jiménez-Moleón,
  • José J. Jiménez-Moleón,
  • Jesús Castilla,
  • Jesús Castilla,
  • Adonina Tardón,
  • Adonina Tardón,
  • Irune Ruiz,
  • Rosana Peiró,
  • Rosana Peiró,
  • Antonio Tejada,
  • María D. Chirlaque,
  • María D. Chirlaque,
  • María D. Chirlaque,
  • Julia A. Butt,
  • Rocío Olmedo-Requena,
  • Rocío Olmedo-Requena,
  • Rocío Olmedo-Requena,
  • Inés Gómez-Acebo,
  • Inés Gómez-Acebo,
  • Pedro Linares,
  • Elena Boldo,
  • Elena Boldo,
  • Elena Boldo,
  • Antoni Castells,
  • Antoni Castells,
  • Antoni Castells,
  • Antoni Castells,
  • Michael Pawlita,
  • Gemma Castaño-Vinyals,
  • Gemma Castaño-Vinyals,
  • Gemma Castaño-Vinyals,
  • Gemma Castaño-Vinyals,
  • Manolis Kogevinas,
  • Manolis Kogevinas,
  • Manolis Kogevinas,
  • Manolis Kogevinas,
  • Silvia de Sanjosé,
  • Silvia de Sanjosé,
  • Marina Pollán,
  • Marina Pollán,
  • Marina Pollán,
  • Rosa del Campo,
  • Rosa del Campo,
  • Tim Waterboer,
  • Nuria Aragonés,
  • Nuria Aragonés

DOI
https://doi.org/10.3389/fmicb.2017.00888
Journal volume & issue
Vol. 8

Abstract

Read online

Background: Several studies have suggested that Helicobacter pylori (H. pylori) infection is a risk factor for colorectal cancer (CRC), while others have not confirmed this hypothesis. This work aimed to assess the relation of CRC with H. pylori seropositivity and with seropositivity to 16 H. pylori proteins, in the MultiCase-Control study, MCC-Spain.Methods: MCC-Spain is a multicase-control study carried out in Spain from 2008 to 2013. In total, 2,140 histologically-confirmed incident CRC cases and 4,098 population-based controls were recruited. Controls were frequency-matched by sex, age, and province. Epidemiological data were collected through a questionnaire fulfilled by face-to-face interviews and a self-administered food-frequency questionnaire. Seroreactivities against 16 H. pylori proteins were determined in 1,488 cases and 2,495 controls using H. pylori multiplex serology. H. pylori seropositivity was defined as positivity to ≥4 proteins. Multivariable logistic regression mixed models were used to estimate odds ratios (OR) and 95% confidence intervals (CI).Results:H. pylori seropositivity was not associated with increased CRC risk (OR = 0.91; 95% CI: 0.71–1.16). Among H. pylori seropositive subjects, seropositivity to Cagδ showed a lower CRC risk, and risk decreased with increasing number of proteins seropositive. Seropositivity to the most recognized virulence factors, CagA and VacA, was not associated with a higher CRC risk. No statistically significant heterogeneity was identified among tumor sites, although inverse relations were stronger for left colon cancer. An interaction with age and sex was found: H. pylori seropositivity was associated with a lower CRC risk in men younger than 65 and with a higher risk in older women.Conclusions: Our results suggest that neither H. pylori seropositivity, nor seropositivity to the virulence factor CagA are associated with a higher CRC risk. A possible effect modification by age and sex was identified.

Keywords