Concerns of resistant markers in marine ecosystem transformed plants

Abstract

World population growth and requirement to global food security, application of genetic engineering and utilization of transgenic organisms have made more important. Using this technology, without regarding to its risks, can cause loses to environment. To generate transgenic organisms, selection systems are applied that cause to selective growth of transformed cells. Antibiotic resistance genes have been found to be very effective for rapidly identifying transformed cell. Because of spreading antibiotic resistant genes or escaping chemical poisons-resistant genes to wild grass species, scientists are looking for ways to generate marker free transgenic organisms or using environmental friendly selectable markers. In the first case, organisms are selected based on antibiotic resistance or chemical poisons resistance, but in next stages selectable marker will be removed. For the second, environmental friendly selectable markers will be used. In the current study, various methods for removing selective marker from transgenic organisms and environmental friendly selectable markers have been proposed which can be used to produce biosafe transgenic organisms.

Keywords

• biosafety
• gene removing
• conformation
• transgenic organisms
• recombination