PROPAGATION OF DUCK PLAGUE VIRUS IN DESI DUCK EMBRYO FIBROBLAST CELLS

Abstract

A virulent strain of duck plague virus isolated from a field outbreak of the disease in Kerala was successfully cultivated in desi duck embryo fibroblast cultures. Though the CPE was not evident during the first two passages, characteristic changes were evident- from the third passage onwards, with rgination of the nuclear chromation and one or more eosinophilic intranuclear .inclusion bodies. The TCIDSo calculated at the 5~' passage was 105.25/ml.