<i>Aspergillus oryzae</i> and <i>Aspergillus niger</i> Co-Cultivation Extract Affects In Vitro Degradation, Fermentation Characteristics, and Bacterial Composition in a Diet-Specific Manner
Fanlin Kong,
Na Lu,
Yanfang Liu,
Shu Zhang,
Hongqin Jiang,
Haomin Wang,
Wei Wang,
Shengli Li
Affiliations
Fanlin Kong
Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, The State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Na Lu
Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, The State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Yanfang Liu
Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, The State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Shu Zhang
Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, The State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Hongqin Jiang
China Representative Office, Ascor Chimici S.R.L., 201199 Bologna, Italy
Haomin Wang
China Representative Office, Ascor Chimici S.R.L., 201199 Bologna, Italy
Wei Wang
Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, The State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Shengli Li
Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, The State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
AOAN may provide enzymes to improve the digestibility of feeds and enhance rumen fermentation. This study determined the effects of AOAN on digestibility, fermentation characteristics, and bacterial composition using in vitro gas recording fermentation system. A total of 30 mg of AOAN was supplemented into 500 mg of TMR, corn silage, oat hay, and alfalfa hay. Fermentation parameters and bacterial communities were determined after 48 h fermentation, and digestibility was determined after 7, 24, 30, and 48 h fermentation. Gas production and dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) digestibility were significantly increased by AOAN supplementation at 48 h (p p > 0.05). AOAN increased starch digestibility in corn silage (p p p p p Prevotella was increased and Ruminococcus was decreased in TMR, oat hay, and alfalfa hay. In conclusion, results suggest that AOAN has the potential to improve the utilization of diets differently, including providing enzymes with changing microbiota (TMR, oat hay, and alfalfa hay) or providing enzymes alone (corn silage).
