Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreri

Xiangyi Zeng; Zujing Yang; Mingyi Sui; Zhi Liu; Qiang Xing; Liping Hu; Liming Jiang; Zhenmin Bao; Xiaoting Huang

Aquaculture Reports (Mar 2025)

Rapid and noninvasive assessment of adductor muscle performance through shell-closing strength of Zhikong scallop Chlamys farreri

Xiangyi Zeng,
Zujing Yang,
Mingyi Sui,
Zhi Liu,
Qiang Xing,
Liping Hu,
Liming Jiang,
Zhenmin Bao,
Xiaoting Huang

Affiliations

Xiangyi Zeng: MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China
Zujing Yang: MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China
Mingyi Sui: MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China
Zhi Liu: MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China
Qiang Xing: MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong 266237, China
Liping Hu: Yantai Marine Economic Research Institute, Yantai, China
Liming Jiang: Yantai Marine Economic Research Institute, Yantai, China
Zhenmin Bao: MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong 266237, China; Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering, Sanya Oceanographic Institution, Ocean University of China, Sanya 572000, China
Xiaoting Huang: MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, Shandong 266237, China; Corresponding author at: MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, China.

Journal volume & issue: Vol. 40
p. 102589

Abstract

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Shell-closing strength (SCS) is a crucial trait of adductor muscle closely associated with the bivalve's swimming ability and health status. However, the investigation and practical application of SCS have been seriously hampered due to the limited availability and complexity of current measurement methodologies. In this study, a rapid and noninvasive method was developed to quantify SCS with four indicators including total force (TF), time of shell closing (TSC), average force (AF), and maximum force (MF) using a self-developed force gauge device. The optimal measurement time and distance for SCS were three minutes and 0.75 cm, respectively. For ten SCS tests, the coefficients of variation (CV) for all four indicators were between 2.54 % and 5.57 %, and the intraclass correlation coefficients (ICC) exceeded 0.75 in each case, which proved that the measurement results were stable and reliable. Subsequent analysis revealed that the four SCS indicators of scallops were significantly associated with the adductor muscle weight (AMW) and the adductor muscle glycogen content (AMG). The correlation coefficients with AMW were 0.609, 0.258, 0.586, and 0.467 for TF, TSC, AF, and MF, respectively (P < 0.05). For AMG, the correlation coefficients were 0.586, 0.372, 0.506, and 0.334 for TF, TSC, AF, and MF, respectively (P < 0.01). The SCS indicators were then used in a ridge regression multivariate linear model to estimate the contents of AMW and AMG in scallops, yielding coefficients of determination (R2) of 0.81 and 0.87, respectively. Furthermore, scallops with stronger SCS were proved to have longer survival times under heat stress, with correlation coefficients ranging from 0.455 to 0.619 (P < 0.01). This finding indicated that SCS could serve as a reliable metric for assessing the heat resistance of scallops. In conclusion, this study provides a quick, accurate, and noninvasive method for measuring the SCS, which could be useful for the evaluation of adductor muscle performance and the selection breeding of scallops for enhanced quality and resistance traits.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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