Characterization of M. laxa and M. fructigena isolates from Hungary with MP-PCR

Abstract

Monilinia laxa (Monilia laxa), Monilinia fructicola (Monilia fructicola) and Monilinia fructigena (Monilia fructigena) are the causal agents of brown rot on pome and stone fruits in Hungary. Forty-five isolates collected from different hosts, different years in several orchards were used for characterization of the M. laxa and M. fructigena population in Hungary. The isolates were identified on species level based on morphological and molecular biological methods; out of these 24 were M. laxa, 20 were M. fructigena and 1 was M. fructicola. Populations of the three Monilinia species were studied with microsatellite primers and the degree of genetic diversity within the species was measured. The population structure analysis revealed that genetic diversity within M. laxa subpopulations was HS= 0.1599, while within M. fructigena subpopulations was HS= 0.2551. The total genetic diversity was HT= 0.3846, while genetic diversity between M. laxa and M. fructigena subpopulations was DST= 0.1771. No clustering relationship was observed among isolates by the different years or hosts.

Keywords

• brown rot
• intraspecific diversity
• microsatellite primers