Nature Communications (Nov 2020)
A catalogue of biochemically diverse CRISPR-Cas9 orthologs
- Giedrius Gasiunas,
- Joshua K. Young,
- Tautvydas Karvelis,
- Darius Kazlauskas,
- Tomas Urbaitis,
- Monika Jasnauskaite,
- Mantvyda M. Grusyte,
- Sushmitha Paulraj,
- Po-Hao Wang,
- Zhenglin Hou,
- Shane K. Dooley,
- Mark Cigan,
- Clara Alarcon,
- N. Doane Chilcoat,
- Greta Bigelyte,
- Jennifer L. Curcuru,
- Megumu Mabuchi,
- Zhiyi Sun,
- Ryan T. Fuchs,
- Ezra Schildkraut,
- Peter R. Weigele,
- William E. Jack,
- G. Brett Robb,
- Česlovas Venclovas,
- Virginijus Siksnys
Affiliations
- Giedrius Gasiunas
- CasZyme
- Joshua K. Young
- Department of Molecular Engineering, Corteva Agriscience™
- Tautvydas Karvelis
- Institute of Biotechnology, Vilnius University
- Darius Kazlauskas
- Institute of Biotechnology, Vilnius University
- Tomas Urbaitis
- CasZyme
- Monika Jasnauskaite
- CasZyme
- Mantvyda M. Grusyte
- CasZyme
- Sushmitha Paulraj
- Department of Molecular Engineering, Corteva Agriscience™
- Po-Hao Wang
- Department of Molecular Engineering, Corteva Agriscience™
- Zhenglin Hou
- Department of Molecular Engineering, Corteva Agriscience™
- Shane K. Dooley
- Department of Agricultural and Biosystems Engineering, Iowa State University
- Mark Cigan
- Department of Molecular Engineering, Corteva Agriscience™
- Clara Alarcon
- Department of Molecular Engineering, Corteva Agriscience™
- N. Doane Chilcoat
- Department of Molecular Engineering, Corteva Agriscience™
- Greta Bigelyte
- Institute of Biotechnology, Vilnius University
- Jennifer L. Curcuru
- New England Biolabs
- Megumu Mabuchi
- New England Biolabs
- Zhiyi Sun
- New England Biolabs
- Ryan T. Fuchs
- New England Biolabs
- Ezra Schildkraut
- New England Biolabs
- Peter R. Weigele
- New England Biolabs
- William E. Jack
- New England Biolabs
- G. Brett Robb
- New England Biolabs
- Česlovas Venclovas
- Institute of Biotechnology, Vilnius University
- Virginijus Siksnys
- CasZyme
- DOI
- https://doi.org/10.1038/s41467-020-19344-1
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 10
Abstract
A few bacterial Cas9 nucleases have been repurposed as genome editing tools. Here, the authors use bioinformatic and biochemical analyses to characterize 79 Cas9 proteins, revealing substantial functional diversity and thus expanding the available toolbox of RNA-programmable CRISPR-associated nucleases.
