Nature Communications (Jul 2020)
Comprehensive structure-function characterization of DNMT3B and DNMT3A reveals distinctive de novo DNA methylation mechanisms
- Linfeng Gao,
- Max Emperle,
- Yiran Guo,
- Sara A. Grimm,
- Wendan Ren,
- Sabrina Adam,
- Hidetaka Uryu,
- Zhi-Min Zhang,
- Dongliang Chen,
- Jiekai Yin,
- Michael Dukatz,
- Hiwot Anteneh,
- Renata Z. Jurkowska,
- Jiuwei Lu,
- Yinsheng Wang,
- Pavel Bashtrykov,
- Paul A. Wade,
- Gang Greg Wang,
- Albert Jeltsch,
- Jikui Song
Affiliations
- Linfeng Gao
- Environmental Toxicology Graduate Program, University of California
- Max Emperle
- Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
- Yiran Guo
- Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill School of Medicine
- Sara A. Grimm
- Division of Intramural Research, Epigenetics and Stem Cell Biology Laboratory, National Institute of Environmental Health Sciences
- Wendan Ren
- Department of Biochemistry, University of California
- Sabrina Adam
- Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
- Hidetaka Uryu
- Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill School of Medicine
- Zhi-Min Zhang
- Department of Biochemistry, University of California
- Dongliang Chen
- Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill School of Medicine
- Jiekai Yin
- Environmental Toxicology Graduate Program, University of California
- Michael Dukatz
- Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
- Hiwot Anteneh
- Department of Biochemistry, University of California
- Renata Z. Jurkowska
- Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
- Jiuwei Lu
- Department of Biochemistry, University of California
- Yinsheng Wang
- Environmental Toxicology Graduate Program, University of California
- Pavel Bashtrykov
- Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
- Paul A. Wade
- Division of Intramural Research, Epigenetics and Stem Cell Biology Laboratory, National Institute of Environmental Health Sciences
- Gang Greg Wang
- Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill School of Medicine
- Albert Jeltsch
- Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University of Stuttgart
- Jikui Song
- Environmental Toxicology Graduate Program, University of California
- DOI
- https://doi.org/10.1038/s41467-020-17109-4
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 14
Abstract
In mammals, DNA methylation patterns are established by two de novo DNA methyltransferases, DNMT3A and DNMT3B. Here the authors report the crystal structures of DNMT3B in complex with both CpG and CpA DNA, providing insight into the substrate-recognition mechanism underpinning the divergent genomic methylation activities of DNMT3A and DNMT3B.
