Functional Analysis of Human Sodium-Phosphate Transporter 4 (NPT4/SLC17A3) Polymorphisms

Promsuk Jutabha; Naohiko Anzai; Toru Kimura; Atsuo Taniguchi; Wako Urano; Hisashi Yamanaka; Hitoshi Endou; Hiroyuki Sakurai

doi:10.1254/jphs.10228sc

Journal of Pharmacological Sciences (Jan 2011)

Functional Analysis of Human Sodium-Phosphate Transporter 4 (NPT4/SLC17A3) Polymorphisms

Promsuk Jutabha,
Naohiko Anzai,
Toru Kimura,
Atsuo Taniguchi,
Wako Urano,
Hisashi Yamanaka,
Hitoshi Endou,
Hiroyuki Sakurai

Affiliations

Promsuk Jutabha: Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo 181-8611, Japan
Naohiko Anzai: Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo 181-8611, Japan; Corresponding author. [email protected]
Toru Kimura: Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo 181-8611, Japan
Atsuo Taniguchi: Institute of Rheumatology, Tokyo Women’s Medical University, Tokyo 162-0054, Japan
Wako Urano: Institute of Rheumatology, Tokyo Women’s Medical University, Tokyo 162-0054, Japan
Hisashi Yamanaka: Institute of Rheumatology, Tokyo Women’s Medical University, Tokyo 162-0054, Japan
Hitoshi Endou: Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo 181-8611, Japan
Hiroyuki Sakurai: Department of Pharmacology and Toxicology, Kyorin University School of Medicine, Tokyo 181-8611, Japan

DOI: https://doi.org/10.1254/jphs.10228sc
Journal volume & issue: Vol. 115, no. 2
pp. 249 – 253

Abstract

Read online

We analyzed the functional properties of five nonsynonymous single nucleotide polymorphisms (SNPs) in the sodium-phosphate transporter NPT4 gene (SLC17A3) using the Xenopus oocyte expression system. NPT4 variants carrying SNP V257F, G279R, or P378L exhibited reduced transport of [14C]para-aminohippurate, [3H]bumetanide, [3H]estrone sulfate, and [14C]urate, when each variant clone was expressed in the plasma membrane of oocytes. This study suggests the possibility that the genetic variation of NPT4 contributes to inter-individual differences in disposition of anionic drugs such as diuretics as well as certain endogenous organic anions such as urate. Keywords:: single nucleotide polymorphism, diuretic, urate

Published in Journal of Pharmacological Sciences

ISSN: 1347-8613 (Print)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.sciencedirect.com/journal/journal-of-pharmacological-sciences

About the journal