Armaghane Danesh Bimonthly Journal (Oct 2020)
The Effect of Catechin Metabolites Derived from Intestinal Microbiota on Ca SKi Cell Proliferation
Abstract
Background & aim: Cervical cancer is the second leading cause of death in women in Iran. Papillomavirus infection is the most important risk factor for developing cervical cancer. Ca SKi cell is a human cervical carcinoma cell that attaches the papillomavirus type 16 to its genome. Catechin is a phenol and a secondary metabolite of a plant. The aim of this study was to determine the effect of catechin metabolites derived from intestinal microbiota on the proliferative capacity of Ca SKi cell line. Methods: In the present in vitro experimental study, catechin metabolites were extracted from fecal samples containing intestinal microbiota by HPLC method and cell line was treated with different concentrations of catechin for 24 and 48 hours. The cytotoxic effects of Ca SKi bacterial cell extract were investigated by MTT assay. After RNA extraction and cDNA preparation, the expression of bax, bcl2 gene in Ca SKi cell line was examined by Real Time PCR. Data were analyzed using one-way analysis of variance Results: No significant associaton was obsereved with increasing bax gene expression and the effective dose of catechin in the first 72 hours (p> 0.05), but with increasing dose effect, a significant increase in bax gene expression was seen. The results indicated that the expression of bcl2 gene was significantly related to the increase in the effective dose of catechin in the first 72 hours. This change was decreasing and had a direct relationship with the effective dose. Conclusion: Catechin may increase the expression of BCL2 in cancer cells, which could be used as a treatment as well as prevention of cervical cancer and bax gene as a prognosis in cervical cancer to develop a new therapeutic effect.
