FEBS Open Bio (May 2020)
Iron overload inhibits self‐renewal of human pluripotent stem cells via DNA damage and generation of reactive oxygen species
- Zhenbo Han,
- Zihang Xu,
- Lei Chen,
- Danyu Ye,
- Yang Yu,
- Ying Zhang,
- Yang Cao,
- Bamba Djibril,
- Xiaofei Guo,
- Xinlu Gao,
- Wenwen Zhang,
- Meixi Yu,
- Shenzhen Liu,
- Gege Yan,
- Mengyu Jin,
- Qi Huang,
- Xiuxiu Wang,
- Bingjie Hua,
- Chao Feng,
- Fan Yang,
- Wenya Ma,
- Yu Liu
Affiliations
- Zhenbo Han
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Zihang Xu
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Lei Chen
- Beijing Ruihua Heart Rehabilitation Research Center China
- Danyu Ye
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Yang Yu
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Ying Zhang
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Yang Cao
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Bamba Djibril
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Xiaofei Guo
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Xinlu Gao
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Wenwen Zhang
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Meixi Yu
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Shenzhen Liu
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Gege Yan
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Mengyu Jin
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Qi Huang
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Xiuxiu Wang
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Bingjie Hua
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Chao Feng
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Fan Yang
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Wenya Ma
- Department of Pharmacy The Affiliated Second Hospital Harbin Medical University Harbin China
- Yu Liu
- Department of Clinical Laboratory Fourth Affiliated Hospital of Harbin Medical University China
- DOI
- https://doi.org/10.1002/2211-5463.12811
- Journal volume & issue
-
Vol. 10,
no. 5
pp. 726 – 733
Abstract
Iron overload affects the cell cycle of various cell types, but the effect of iron overload on human pluripotent stem cells has not yet been reported. Here, we show that the proliferation capacities of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were significantly inhibited by ferric ammonium citrate (FAC) in a concentration‐dependent manner. In addition, deferoxamine protected hESCs/hiPSCs against FAC‐induced cell‐cycle arrest. However, iron overload did not affect pluripotency in hESCs/hiPSCs. Further, treatment of hiPSCs with FAC resulted in excess reactive oxygen species production and DNA damage. Collectively, our findings provide new insights into the role of iron homeostasis in the maintenance of self‐renewal in human pluripotent stem cells.
Keywords
- cell cycle
- DNA damage
- human embryonic stem cells
- human induced pluripotent stem cells
- iron overload
- ROS
