Frontiers in Plant Science (May 2021)

Genome-Wide Analysis of DA1-Like Genes in Gossypium and Functional Characterization of GhDA1-1A Controlling Seed Size

  • Shuxian Yang,
  • Shuxian Yang,
  • Li Huang,
  • Jikun Song,
  • Lisen Liu,
  • Yingying Bian,
  • Bing Jia,
  • Luyao Wu,
  • Luyao Wu,
  • Yue Xin,
  • Man Wu,
  • Jinfa Zhang,
  • Jiwen Yu,
  • Jiwen Yu,
  • Xinshan Zang,
  • Xinshan Zang

DOI
https://doi.org/10.3389/fpls.2021.647091
Journal volume & issue
Vol. 12

Abstract

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Cotton (Gossypium spp.) is an economically important crop grown for natural fiber and seed oil production. DA1 is a ubiquitin receptor that determines final seed and organ size by restricting the period of cell proliferation. In the present study, we identified 7 DA1-like genes each in cultivated tetraploid (AADD) G. hirsutum and G. barbadense, and 4 and 3 DA1-like genes in their ancestral diploid G. arboreum (A2A2) and G. raimondii (D5D5), respectively. The 7 GhDA1 genes were confirmed to be distributed on four At and three Dt subgenome chromosomes in G. hirsutum. GhDA1-1A showed a high sequence similarity to AtDA1 in Arabidopsis, and they possessed the same functional domains, suggesting conserved functions. The overexpression of GhDA1-1AR301K in Arabidopsis significantly increased seed size and seed weight, indicating that GhDA1-1A is a promising target for cotton improvement. This study provides information on the molecular evolutionary properties of DA1-like genes in cotton, which will be useful for the genetic improvement of cotton.

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