Discussion on the establishment of blood glucose fluctuation animal models

Abstract

AIM: To provide the experimental basis for the in vivo study of blood glucose fluctuation injury mechanism, through intraperitoneal injection of glucose to establish blood glucose fluctuation animal models and to simulate blood glucose fluctuation of patients with diabetes.METHODS: Rats were randomly divided into four groups: normal control group(NC), normal fluctuation group(NF), diabetes mellitus group(DM)and diabetes fluctuation group(DF). Diabetic models were induced through intraperitoneal injection of STZ. A certain amount of glucose was injected in the rats of group NF and DF intraperitoneally three times a day after the model was established, thereby causing blood glucose fluctuations. Full-day blood glucose monitoring was conducted once every two weeks to observe the blood glucose fluctuation situation, according to blood glucose levels drawing the “time-blood glucose concentration” curve. The stability of blood glucose was evaluated from four different aspects, through calculating mean blood glucose(MBG), standard deviation blood glucose(SDBG), largest amplitude of glycemic excursions(LAGE)and Sclichtkrull Mz value.RESULTS: The full-day blood glucose of rats in the group NC was in a normal range, and it was stable without fluctuation. The blood glucose of rats in group NF was fluctuated between 5-10mmol/L. The blood glucose of rats in group DM were maintained at higher level(>20mmol/L), without great fluctuation extent and DF with sighificant fluctuation. The blood glucose fluctuation in graoup DF was significant. The blood glucose changes of rats in the group NF and DF were significant and regular. The curve of blood glucose fluctuations was relatively stable. All values of rats in group NF, DM and DF were significantly increased compared with group NC. Group DF was increased more significantly, and the comparison with group NF and DM had significant difference(PCONCLUSION: Significant blood glucose fluctuation can be formed through the method of manual intraperitoneal injection of glucose. The method can be used for simulating the blood glucose fluctuation situation of patients with diabetics. This method has the advantages of simple operation, low injury and good repeatability. It can provide experimental basis for in vivo study of blood glucose fluctuation injury mechanism and pathogenesis of diabetic complications.

Keywords

• diabetes mellitus
• blood glucose fluctuation
• animal model
• animal model