Restoration of Physiological Levels of Uric Acid and Ascorbic Acid Reroutes the Metabolism of Stored Red Blood Cells
Manon Bardyn,
Jingkui Chen,
Michaël Dussiot,
David Crettaz,
Lucas Schmid,
Emmanuel Längst,
Pascal Amireault,
Jean-Daniel Tissot,
Mario Jolicoeur,
Michel Prudent
Affiliations
Manon Bardyn
Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, CH-1066 Epalinges, Switzerland
Jingkui Chen
Département de Génie Chimique, École Polytechnique de Montréal, P.O. Box 6079, Centre-Ville Station, Montréal, QC H3C 3A7, Canada
Michaël Dussiot
INSERM UMR 1163, Laboratory of Cellular and Molecular Mechanisms of Hematological Disorders and Therapeutical Implications, INTS, Université de Paris, 75015 Paris, France
David Crettaz
Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, CH-1066 Epalinges, Switzerland
Lucas Schmid
Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, CH-1066 Epalinges, Switzerland
Emmanuel Längst
Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, CH-1066 Epalinges, Switzerland
Pascal Amireault
INSERM UMR 1163, Laboratory of Cellular and Molecular Mechanisms of Hematological Disorders and Therapeutical Implications, INTS, Université de Paris, 75015 Paris, France
Jean-Daniel Tissot
Centre de Transfusion Sanguine, Faculté de Biologie et de Médicine, Université de Lausanne, CH-1011 Lausanne, Switzerland
Mario Jolicoeur
Département de Génie Chimique, École Polytechnique de Montréal, P.O. Box 6079, Centre-Ville Station, Montréal, QC H3C 3A7, Canada
Michel Prudent
Laboratoire de Recherche sur les Produits Sanguins, Transfusion Interrégionale CRS, CH-1066 Epalinges, Switzerland
After blood donation, the red blood cells (RBCs) for transfusion are generally isolated by centrifugation and then filtrated and supplemented with additive solution. The consecutive changes of the extracellular environment participate to the occurrence of storage lesions. In this study, the hypothesis is that restoring physiological levels of uric and ascorbic acids (major plasmatic antioxidants) might correct metabolism defects and protect RBCs from the very beginning of the storage period, to maintain their quality. Leukoreduced CPD-SAGM RBC concentrates were supplemented with 416 µM uric acid and 114 µM ascorbic acid and stored during six weeks at 4 °C. Different markers, i.e., haematological parameters, metabolism, sensitivity to oxidative stress, morphology and haemolysis were analyzed. Quantitative metabolomic analysis of targeted intracellular metabolites demonstrated a direct modification of several metabolite levels following antioxidant supplementation. No significant differences were observed for the other markers. In conclusion, the results obtained show that uric and ascorbic acids supplementation partially prevented the metabolic shift triggered by plasma depletion that occurs during the RBC concentrate preparation. The treatment directly and indirectly sustains the antioxidant protective system of the stored RBCs.
