Protocol for the cryopreservation of Cryptosporidium isolates using enteroids

Miner Deng; Yanting Wei; Na Li; Lihua Xiao; Yaoyu Feng; Yaqiong Guo

STAR Protocols (Jun 2024)

Protocol for the cryopreservation of Cryptosporidium isolates using enteroids

Miner Deng,
Yanting Wei,
Na Li,
Lihua Xiao,
Yaoyu Feng,
Yaqiong Guo

Affiliations

Miner Deng: State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
Yanting Wei: State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China
Na Li: State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
Lihua Xiao: State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Corresponding author
Yaoyu Feng: State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Corresponding author
Yaqiong Guo: State Key Laboratory for Animal Disease Control and Prevention, South China Agricultural University, Guangzhou, Guangdong 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Center for Emerging and Zoonotic Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China; Corresponding author

Journal volume & issue: Vol. 5, no. 2
p. 103101

Abstract

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Summary: A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids. We describe the steps for the establishment of enteroid cultures and cryopreservation of C. parvum-infected HCT-8 cultures. We then detail procedures for the recovery and propagation of frozen parasites using enteroids.For complete details on the use and execution of this protocol, please refer to Deng et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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