Site-specific identification and quantitation of endogenous SUMO modifications under native conditions

Ryan J. Lumpkin; Hongbo Gu; Yiying Zhu; Marilyn Leonard; Alla S. Ahmad; Karl R. Clauser; Jesse G. Meyer; Eric J. Bennett; Elizabeth A. Komives

doi:10.1038/s41467-017-01271-3

Nature Communications (Oct 2017)

Site-specific identification and quantitation of endogenous SUMO modifications under native conditions

Ryan J. Lumpkin,
Hongbo Gu,
Yiying Zhu,
Marilyn Leonard,
Alla S. Ahmad,
Karl R. Clauser,
Jesse G. Meyer,
Eric J. Bennett,
Elizabeth A. Komives

Affiliations

Ryan J. Lumpkin: Department of Chemistry and Biochemistry, University of California, San Diego
Hongbo Gu: Proteomic Service Group Cell Signaling Technology
Yiying Zhu: Proteomic Service Group Cell Signaling Technology
Marilyn Leonard: Cell and Developmental Biology, University of California, San Diego
Alla S. Ahmad: Department of Chemistry and Biochemistry, University of California, San Diego
Karl R. Clauser: Broad Institute of MIT and Harvard
Jesse G. Meyer: Department of Chemistry and Biochemistry, University of California, San Diego
Eric J. Bennett: Cell and Developmental Biology, University of California, San Diego
Elizabeth A. Komives: Department of Chemistry and Biochemistry, University of California, San Diego

DOI: https://doi.org/10.1038/s41467-017-01271-3
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 11

Abstract

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SUMOylation is post-translational modification implicated in several biological pathways. Here the authors describe an approach for the global profiling of SUMO attachment sites under native conditions that also allows the parallel determination of SUMO and Ub attachments.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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