Isolation and Identification of Lipopeptide-Producing <i>Bacillus velezensis</i> Strains from Wheat Phyllosphere with Antifungal Activity against the Wheat Pathogen <i>Zymoseptoria tritici</i>
Rémi Platel,
Mélodie Sawicki,
Qassim Esmaeel,
Béatrice Randoux,
Pauline Trapet,
Mohammed El Guilli,
Noureddine Chtaina,
Ségolène Arnauld,
Alexandre Bricout,
Alice Rochex,
Natacha Bourdon,
Patrice Halama,
Cédric Jacquard,
Essaid Ait Barka,
Philippe Reignault,
Maryline Magnin-Robert,
Ali Siah
Affiliations
Rémi Platel
Joint Research Unit 1158 BioEcoAgro, Junia, University of Lille, INRAE, University of Liège, UPJV, University of Artois, ULCO, 59000 Lille, France
Mélodie Sawicki
Unité de Chimie Environnementale et Interactions sur le Vivant (EA 4492), Université du Littoral Côte d’Opale, CS 80699 CEDEX, 62228 Calais, France
Qassim Esmaeel
Unité de Recherche Résistance Induite et Bio-Protection des Plantes-EA 4707—USC INRAe1488, Université de Reims Champagne-Ardenne, 51100 Reims, France
Béatrice Randoux
Unité de Chimie Environnementale et Interactions sur le Vivant (EA 4492), Université du Littoral Côte d’Opale, CS 80699 CEDEX, 62228 Calais, France
Pauline Trapet
Joint Research Unit 1158 BioEcoAgro, Junia, University of Lille, INRAE, University of Liège, UPJV, University of Artois, ULCO, 59000 Lille, France
Mohammed El Guilli
Center of Agricultural Research of Kenitra 14070, Plant Pathology and Post-Harvest Quality, National Institute of Agricultural Research, Kenitra 14070, Morocco
Noureddine Chtaina
Laboratory of Phytopathology, Department of Production, Protection and Biotechnology, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat 10010, Morocco
Ségolène Arnauld
Joint Research Unit 1158 BioEcoAgro, Junia, University of Lille, INRAE, University of Liège, UPJV, University of Artois, ULCO, 59000 Lille, France
Alexandre Bricout
Joint Research Unit 1158 BioEcoAgro, Junia, University of Lille, INRAE, University of Liège, UPJV, University of Artois, ULCO, 59000 Lille, France
Alice Rochex
Joint Research Unit 1158 BioEcoAgro, Junia, University of Lille, INRAE, University of Liège, UPJV, University of Artois, ULCO, 59000 Lille, France
Natacha Bourdon
Unité de Chimie Environnementale et Interactions sur le Vivant (EA 4492), Université du Littoral Côte d’Opale, CS 80699 CEDEX, 62228 Calais, France
Patrice Halama
Joint Research Unit 1158 BioEcoAgro, Junia, University of Lille, INRAE, University of Liège, UPJV, University of Artois, ULCO, 59000 Lille, France
Cédric Jacquard
Unité de Recherche Résistance Induite et Bio-Protection des Plantes-EA 4707—USC INRAe1488, Université de Reims Champagne-Ardenne, 51100 Reims, France
Essaid Ait Barka
Unité de Recherche Résistance Induite et Bio-Protection des Plantes-EA 4707—USC INRAe1488, Université de Reims Champagne-Ardenne, 51100 Reims, France
Philippe Reignault
Unité de Chimie Environnementale et Interactions sur le Vivant (EA 4492), Université du Littoral Côte d’Opale, CS 80699 CEDEX, 62228 Calais, France
Maryline Magnin-Robert
Unité de Chimie Environnementale et Interactions sur le Vivant (EA 4492), Université du Littoral Côte d’Opale, CS 80699 CEDEX, 62228 Calais, France
Ali Siah
Joint Research Unit 1158 BioEcoAgro, Junia, University of Lille, INRAE, University of Liège, UPJV, University of Artois, ULCO, 59000 Lille, France
Septoria tritici blotch, caused by the fungal pathogen Zymoseptoria tritici, is a highly significant disease on wheat crops worldwide. The objective of the present study was to find out new bacterial strains with bio-antimicrobial activity against Z. tritici. Two phyllospheric bacteria (S1 and S6) were isolated from wheat ears and identified as Bacillus velezensis strains according to 16S rRNA Sanger sequencing. Antagonistic assays performed with either living strains or cell-free culture filtrates showed significant in vitro antifungal activities against Z. tritici. For the culture filtrates, the half-maximal inhibitory dilution and the minimal inhibitory dilution were 1.4% and 3.7% for the strain S1, and 7.4% and 15% for the strain S6, respectively. MALDI—ToF analysis revealed that both strains synthesize cyclic lipopeptides but from different families. Interestingly, only strain S1 produces putative bacillomycin D. Such differential lipopeptide production patterns might explain the difference observed between the antifungal activity of the culture filtrates of the two strains. This study allows the identification of new lipopeptide-producing strains of B. velezensis with a high potential of application for the biocontrol of Z. tritici.
