Biological control of a novel strain Bacillus velezensis CMML21–47 against sweet potato wilt and black rot diseases

Yoeng-Seok Yoon; Ju Gyeong Lee; Narayan Chandra Paul; Soyoon Park; Sojung Ryu; Hyunsook Kim; Hyeong Woo Kim; Seonghun Im; Hyunkyu Sang; Gui Hwan Han

Biological Control (Aug 2024)

Biological control of a novel strain Bacillus velezensis CMML21–47 against sweet potato wilt and black rot diseases

Yoeng-Seok Yoon,
Ju Gyeong Lee,
Narayan Chandra Paul,
Soyoon Park,
Sojung Ryu,
Hyunsook Kim,
Hyeong Woo Kim,
Seonghun Im,
Hyunkyu Sang,
Gui Hwan Han

Affiliations

Yoeng-Seok Yoon: Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup 56212, Republic of Korea; Food Safety and Processing Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea
Ju Gyeong Lee: Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea
Narayan Chandra Paul: Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea; Kumho Life Science Laboratory, Chonnam National University, Gwangju 61186, Republic of Korea
Soyoon Park: Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea
Sojung Ryu: Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup 56212, Republic of Korea
Hyunsook Kim: Boran Pharma, Seoul 04206, Republic of Korea
Hyeong Woo Kim: Boran Pharma, Seoul 04206, Republic of Korea
Seonghun Im: Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup 56212, Republic of Korea
Hyunkyu Sang: Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea; Kumho Life Science Laboratory, Chonnam National University, Gwangju 61186, Republic of Korea; Corresponding authors at: Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea (H. Sang), Center for Industrialization of Agriculture and Livestock Microorganisms, Jeongeup 56212, Republic of Korea (G.H. Han).
Gui Hwan Han: Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup 56212, Republic of Korea; Corresponding authors at: Department of Integrative Food, Bioscience and Biotechnology, Chonnam National University, Gwangju 61186, Republic of Korea (H. Sang), Center for Industrialization of Agriculture and Livestock Microorganisms, Jeongeup 56212, Republic of Korea (G.H. Han).

Journal volume & issue: Vol. 195
p. 105541

Abstract

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A root bacterium identified as Bacillus velezensis CMML21–47 with strong biocontrol activity against two major sweet potato fungal diseases caused by Fusarium oxysporum and Ceratocystis fimbriata was isolated in this study. In vitro bioassays and field assays were conducted to test the biocontrol activity and confirmed its strong antagonistic activity. The complete genome of B. velezensis CMML21–47 was sequenced, and multiple gene clusters related to the synthesis of antibiotics by antiSMASH analysis were detected. Selective ultra–high performance liquid chromatography with quadrupole time–of–flight mass spectrometry (UPLC–Q–TOF–MS) was performed and detected lipopeptides, including fengycin, surfactin, and bacillomycin. Lipopeptide fractions were separated, tested against the pathogens, and showed strong growth inhibition. Accordingly, CMML21-47 is an attractive antifungal agent, with high potential to control the sweet potato pathogens causing wilt and black rot diseases.

Published in Biological Control

ISSN: 1049-9644 (Print); 1090-2112 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Agriculture; Science: Biology (General)
Website: https://www.sciencedirect.com/journal/biological-control

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