Mechanism study of novel inducer combinations containing laver powder for Penicillium piceum lignocellulolytic enzyme production
Wendi Jia,
Mingyue Ge,
Zhaokun Zhang,
Dongyuan Zhang,
Le Gao
Affiliations
Wendi Jia
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesNational Technology Innovation Center of Synthetic Biology Tianjin China
Mingyue Ge
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesNational Technology Innovation Center of Synthetic Biology Tianjin China
Zhaokun Zhang
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesNational Technology Innovation Center of Synthetic Biology Tianjin China
Dongyuan Zhang
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesNational Technology Innovation Center of Synthetic Biology Tianjin China
Le Gao
Tianjin Key Laboratory for Industrial BioSystems and Bioprocessing Engineering Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesNational Technology Innovation Center of Synthetic Biology Tianjin China
Abstract Inducers play an important role in fungus cellulase production. In this study, a combination of Avicel and laver powder, which contains cellulose, hemicellulose, other polysaccharides, and crude lipid, was used to induce lignocellulolytic enzyme production in Penicillium piceum H16. When Avicel was supplemented with various amounts of laver powder, cellulase activities or extracellular protein yields increased by 11.4%–102.5%, relative to those observed when Avicel alone was used. In particular, filter paper and cellobiohydrolase activities increased by as much as 39.3% and 102.5%, respectively, after supplementation with laver powder. Furthermore, laver powder supplementation enhanced the efficiency of cellulose and hemicellulose hydrolysis in crude corn stover by 39.3% and 22.2%, respectively. The results suggest that laver powder and Avicel synergistically induce lignocellulytic enzyme production and P. piceum H16 growth. iTRAQ‐based quantitative proteomics analysis results further demonstrate that laver powder supplementation optimized the synergistic lignocellulytic enzyme cocktail from P. piceum H16 by upregulating the production of cellulase‐enhancing factors and lignocellulolytic enzymes. The oligosaccharides released by laver powder may account for the synergistic induction, which may help reduce the cost of producing lignocellulytic enzymes by filamentous fungi.
