UPLC-QToF-ESI-MS identification and anthelmintic activity of Mitragyna inermis (Willd.) Kuntze (Rubiaceae)

Placide Mahougnan Toklo; Géorcelin G. Alowanou; Steven Collins N. Wouamba; Fidèle M. Assogba; Mathias A. Ahomadegbe; Amoussatou Sakirigui; Bruno Ndjakou Lenta; Sylvie Hounzangbe-Adote; Simeon Fogue Kouam; Eléonore C. Yayi-Ladekan; Joachim Djimon Gbenou

Heliyon (Jun 2023)

UPLC-QToF-ESI-MS identification and anthelmintic activity of Mitragyna inermis (Willd.) Kuntze (Rubiaceae)

Placide Mahougnan Toklo,
Géorcelin G. Alowanou,
Steven Collins N. Wouamba,
Fidèle M. Assogba,
Mathias A. Ahomadegbe,
Amoussatou Sakirigui,
Bruno Ndjakou Lenta,
Sylvie Hounzangbe-Adote,
Simeon Fogue Kouam,
Eléonore C. Yayi-Ladekan,
Joachim Djimon Gbenou

Affiliations

Placide Mahougnan Toklo: Laboratoire de Pharmacognosie et des Huiles Essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP: 918, Cotonou, Benin; Laboratoire d’Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d’Abomey Calavi, 01 BP 526, Cotonou, Benin; Department of Chemistry, Higher Teacher Training College, University of Yaounde I, 4124, Yaounde, P.O. Box 47, Yaounde, Cameroon; Corresponding author. Laboratoire de Pharmacognosie et des Huiles Essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP: 918, Cotonou, Benin.
Géorcelin G. Alowanou: Laboratoire d’Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d’Abomey Calavi, 01 BP 526, Cotonou, Benin
Steven Collins N. Wouamba: Department of Chemistry, Higher Teacher Training College, University of Yaounde I, 4124, Yaounde, P.O. Box 47, Yaounde, Cameroon
Fidèle M. Assogba: Laboratoire de Pharmacognosie et des Huiles Essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP: 918, Cotonou, Benin
Mathias A. Ahomadegbe: Laboratoire de Pharmacognosie et des Huiles Essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP: 918, Cotonou, Benin
Amoussatou Sakirigui: Laboratoire de Pharmacognosie et des Huiles Essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP: 918, Cotonou, Benin
Bruno Ndjakou Lenta: Department of Chemistry, Higher Teacher Training College, University of Yaounde I, 4124, Yaounde, P.O. Box 47, Yaounde, Cameroon
Sylvie Hounzangbe-Adote: Laboratoire d’Ethnopharmacologie et de Santé Animale, Faculté des Sciences Agronomiques, Université d’Abomey Calavi, 01 BP 526, Cotonou, Benin
Simeon Fogue Kouam: Department of Chemistry, Higher Teacher Training College, University of Yaounde I, 4124, Yaounde, P.O. Box 47, Yaounde, Cameroon
Eléonore C. Yayi-Ladekan: Laboratoire de Pharmacognosie et des Huiles Essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP: 918, Cotonou, Benin
Joachim Djimon Gbenou: Laboratoire de Pharmacognosie et des Huiles Essentielles, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, 01 BP: 918, Cotonou, Benin; Corresponding author.

Journal volume & issue: Vol. 9, no. 6
p. e16448

Abstract

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Medicinal plants attract the attention of many researchers to find natural and safe remedies for various resistant diseases. Leaves of Mitragyna inermis are widely used in traditional veterinary medicine for the treatment of gastrointestinal strongyles of small ruminants. The aim of the current study is to estimate the antioxidant, anthelmintic and the larval toxicity of the aqueous and hydroethanolic extracts of this plant in addition to the hexane, dichloromethane and ethanol fractions of the hydroethanolic extract. Investigation of the most active extract using Ultra Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Electrospray Ionization Mass Spectrometry (UPLC-QToF-ESI-MS). Both plant extracts showed good antioxidant activity by scavenging the 2,2′-diphényl-1-picrylhydrazyl (DPPH) radical and reducing the ferric ion. Similarly, they were no-toxic to Artemia salina larvae (CL50 > 0.1 μg/mL). Also, they significantly reduced larval migration and motility of Haemonchus contortus adult worms (p < 0.001). The hexane, dichloromethane and ethanolic fractions of the hydroethanolic extract showed low activity compared to crude extracts except for the hexane fraction on H. contortus adult worms (p < 0.001) while it showed a poor result on larvae. It thus appears that the anthelmintic activity of the extract may be linked to the synergistic action of these compounds. The UPLC-QToF-ESI-MS analysis revealed the tentative identification of 15 compounds including 7 alkaloids. The results of the present study confirm the anthelmintic activity of M. inermis in traditional veterinary medicine.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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