Salmonella serotyping and molecular typing in meat products in Yibin city of Sichuan Province from 2015 to 2020

Abstract

ObjectiveTo analyze the influencing factors of Salmonella contamination， the serotype distribution and molecular typing of Salmonella isolates in meat products in Yibin city were studied.MethodsA total of 29 Salmonella strains isolated from commercial meat products in Yibin city of from 2015 to 2020 were analyzed by slide agglutination test for serotype and pulsed field gel electrophoresis（PFGE） for molecular subtyping. Multivariate Logistic regression analysis was carried out on the effects of geographical distribution， sample source and sales mode on the detection composition of Salmonella serogroup.ResultsFourteen serotypes and 4 serogroups were detected in the Salmonella isolates. The dominant bacteria were Salmonella typhimurium， Salmonella give and Salmonella enteritidis. The PFGE cluster analysis showed 18 genotype. The genotypes of Salmonella enteritidis were highly homologous with similarity index of 100%， indicating the same source. Salmonella typhimurium with 4 genotypes and Salmonella give with 2 genotypes both had lower similarity. Multivariate Logistic analysis showed that the risk factor on serogroup D of Salmonella was geographical distribution （OR=1.053， P<0.05）. The risk factors on serogroup E of Salmonella were sample source （OR=3.887， P<0.05） and geographical distribution （OR=1.227， P<0.05）.ConclusionThe poultry breeding sites and sample source are risk factors for Salmonella infection， and the hygienic inspection should be reinforced accordingly.

Keywords

• salmonella
• serotype
• molecular typing
• multivariate analysis