Arabidopsis Seed Germination Assay with Gibberellic Acid

Chunmei Zhong; Hao Xu; Siting Ye; Shengchun  Zhang; Xiaojing  Wang

doi:10.21769/BioProtoc.2005

Bio-Protocol (Nov 2016)

Arabidopsis Seed Germination Assay with Gibberellic Acid

Chunmei Zhong,
Hao Xu,
Siting Ye,
Shengchun Zhang,
Xiaojing Wang

Affiliations

Chunmei Zhong: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China
Hao Xu: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China
Siting Ye: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China
Shengchun Zhang: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China
Xiaojing Wang: Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, China

DOI: https://doi.org/10.21769/BioProtoc.2005
Journal volume & issue: Vol. 6, no. 22

Abstract

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This assay analyzes Arabidopsis seed germination in response to gibberellic acid (GA). During seed imbibition, visible physiological changes allow precise determination of germination rate. This protocol utilizes a stereoscopic microscope to improve characterization of seed germination process.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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