Epigenomics, Proliferation, and the Identity of Cells, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Paris, France; Equipe Labellisée LIGUE Contre le Cancer, Paris, France
Thaleia Papadopoulou
Epigenomics, Proliferation, and the Identity of Cells, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Paris, France; Equipe Labellisée LIGUE Contre le Cancer, Paris, France
Nicola Festuccia
Epigenomics, Proliferation, and the Identity of Cells, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Paris, France; Equipe Labellisée LIGUE Contre le Cancer, Paris, France
Alexandra Tachtsidi
Epigenomics, Proliferation, and the Identity of Cells, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Paris, France; Equipe Labellisée LIGUE Contre le Cancer, Paris, France; Sorbonne Université, Collège Doctoral, Paris, France
Inma Gonzalez
Epigenomics, Proliferation, and the Identity of Cells, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Paris, France; Equipe Labellisée LIGUE Contre le Cancer, Paris, France
Agnes Dubois
Epigenomics, Proliferation, and the Identity of Cells, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Paris, France; Equipe Labellisée LIGUE Contre le Cancer, Paris, France
Sandrine Vandormael-Pournin
Epigenomics, Proliferation, and the Identity of Cells, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Paris, France; Early Mammalian Development and Stem Cell Biology, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, Paris, France
Elphège P Nora
Gladstone Institutes, San Francisco, United States; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States
Gladstone Institutes, San Francisco, United States; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States; Department of Pediatrics, University of California, San Francisco, San Francisco, United States
Epigenomics, Proliferation, and the Identity of Cells, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Paris, France; Early Mammalian Development and Stem Cell Biology, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR 3738, Paris, France
Epigenomics, Proliferation, and the Identity of Cells, Department of Developmental and Stem Cell Biology, Institut Pasteur, CNRS UMR3738, Paris, France; Equipe Labellisée LIGUE Contre le Cancer, Paris, France
The access of Transcription Factors (TFs) to their cognate DNA binding motifs requires a precise control over nucleosome positioning. This is especially important following DNA replication and during mitosis, both resulting in profound changes in nucleosome organization over TF binding regions. Using mouse Embryonic Stem (ES) cells, we show that the TF CTCF displaces nucleosomes from its binding site and locally organizes large and phased nucleosomal arrays, not only in interphase steady-state but also immediately after replication and during mitosis. Correlative analyses suggest this is associated with fast gene reactivation following replication and mitosis. While regions bound by other TFs (Oct4/Sox2), display major rearrangement, the post-replication and mitotic nucleosome positioning activity of CTCF is not unique: Esrrb binding regions are also characterized by persistent nucleosome positioning. Therefore, selected TFs such as CTCF and Esrrb act as resilient TFs governing the inheritance of nucleosome positioning at regulatory regions throughout the cell-cycle.
