Drug Design, Development and Therapy (Nov 2023)

Metabolomics Profiles Reveal the Efficacy of Wuzhuyu Decoction on Patients with Chronic Non-Atrophic Gastritis

  • Hu Q,
  • Zeng J,
  • Zhang X,
  • He T,
  • Zhang A,
  • Li J,
  • Wei S,
  • Jing M,
  • Li H,
  • Wang X,
  • Chang L,
  • Ma X,
  • Zhao Y

Journal volume & issue
Vol. Volume 17
pp. 3269 – 3280


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Qichao Hu,1,2,* Jinhao Zeng,3,4,* Xiaomei Zhang,5 Tingting He,6 Aozhe Zhang,6 Jianyu Li,6 Shizhang Wei,2,7 Manyi Jing,2 Haotian Li,2 Xin Wang,2 Lei Chang,2 Xiao Ma,1,2 Yanling Zhao2 1State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, People’s Republic of China; 2Department of Pharmacy, Chinese PLA General Hospital, Beijing, People’s Republic of China; 3TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, People’s Republic of China; 4Department of Gastroenterology, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, 610072, People’s Republic of China; 5Institute of Medicinal Chemistry of Chinese Medicine, Chongqing Academy of Chinese Materia Medica, Chongqing, People’s Republic of China; 6Division of Integrative Medicine, The Fifth Medical Center, General Hospital of PLA, Beijing, People’s Republic of China; 7Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Health Science Center, Peking University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiao Ma; Yanling Zhao, Email [email protected]; [email protected]: Chronic non-atrophic gastritis (CNAG) is a common clinical gastrointestinal disease with a long and recurrent course. In China, Wuzhuyu decoction (WZYD) has been used for centuries to treat gastrointestinal disorders. To unravel the efficacy and mechanism of WZYD for CNAG, a clinical study was conducted. And metabolomics was used to explore the mechanism of WZYD for CNAG patients.Methods: Twenty patients in total were recruited in this study (Nos. ChiCTR2200062296) and the protocol was approved by the Ethics Committee (Approval number: KY-2022-2-6-1) and complied with the Declaration of Helsinki. The formula granule of WZYD were assessed by UHPLC-QQQ-TOF to discern the main potential active compounds. The endoscopy evaluation and histopathological changes were detected as effective indicators. Serum samples from patients were used for metabolomics. Inflammatory factors in patients’ serum were determined by ELISA. Metabolomics revealed a series of differential metabolites and signaling pathways.Results: WZYD was capable to prevent CNAG by ameliorating score of endoscopy evaluation including erosion, hemorrhage, as well as chronic inflammation and active chronic inflammation score after treatment were decreased. The results indicated that 10 core metabolic components were associated with the treatment of WZYD. Moreover, these metabolic components proved that pyrimidine metabolism and thiamine metabolism were critically responsible for CNAG. In addition, WZYD treatment effectively reduced serum levels of TNF-α, IL-10, and COX-2.Conclusion: Altogether, WZYD can effectively alleviate CNAG by inhibiting inflammation and regulating related metabolic processes, which might be the molecular mechanism of WZYD treatment of CNAG. More studies are warranted to be conducted in this area.Trial Registration: ChiCTR, ChiCTR2200062296. Registered 1 August 2022, https://www.chictr.org.cn/com/25/showprojen.aspx?proj=174027.Keywords: Wuzhuyu decoction, chronic non-atrophic gastritis, metabolomics, natural product